清咽滴丸治疗大鼠口腔溃疡的实验研究

Experimental research on Qingyan Droping Pills for treatment of oral ulcer in rats

目的探讨清咽滴丸治疗口腔溃疡的治疗效果。方法 SPF级大鼠60只,随机分为对照组,模型组,清咽滴丸高、中、低剂量(90、30、10 mg/kg)组,阳性药(桂林西瓜霜200 mg/kg)组,每组10只。于大鼠口腔左侧颊部黏膜下注射醋酸制备口腔溃疡模型。造模后第2天开始给药,连续给药10 d。用游标卡尺测量各组大鼠给药前及给药后第4、7、10天的口腔溃疡的直径,计算溃疡面积;取口腔溃疡部位组织,其中一部分溃疡组织制备匀浆,按试剂盒方法测定肿瘤坏死因子-α(TNF-α)水平,剩余溃疡组织切片观察溃疡组织形态变化。结果高剂量清咽滴丸组和桂林西瓜霜组大鼠口腔溃疡面积均显著减小(P<0.05、0.01),溃疡部位组织病变明显减轻,TNF-α水平显著降低(P<0.01)。结论清咽滴丸对醋酸诱导的口腔溃疡具有一定治疗作用。

Objective To study the treatment effect of Qingyan Droping Pills（QDP） for oral ulcer. Methods SPF level 60 rats were randomly divided into control group, model group, positive control（Guilin Watermelon Frost 200 mg/kg） group, high-, mid-, and low-dose QDP groups（n = 10）. The oral ulcer model of rat was established by injection of acetic acid on the oral mucosa of left cheek in rat, at 24 h immediately after the formation of ulcers. The first two days after the start of the administration modeling, continuous administration of 10 d was performed. The ulcer diameters before the administration and 4, 7, 10 d after administration were recorded by vernier caliper. Rats in each group after the last measurement were sacrificed and part of oral ulcers were removed, some of ulcers tissue were homogenized by TNF-α assay kit. The remaining ulcers were sliced and the ulcer morphology was observed. Results Mouth ulcer areas of high-dose QDP group and Watermelon Frost group were significantly reduced（P 〈0.05, 0.01）, ulcer tissue lesions and TNF-α levels were significantly reduced（P 〈0.01）. Conclusion QDP on acetic acid-induced oral ulcers has some therapeutic effect.