维吾尔医学异常黑胆质体液结肠癌患者肠道菌群的分布情况研究

Analysis of intestinal bacterial diversity in abnormal sawda hilit people of colon cancer in traditional uyghur medicine

目的通过先进的分子生物学方法分析维吾尔医学异常黑胆质结肠癌患者肠道菌群分布情况及多样性。方法对结肠癌患者进行维吾尔医学体液分型并挑取其中异常黑胆质患者,采集患者粪便样品,提取细菌总DNA并设计一对通用引物扩增16SrDNA的V6-V8可变区进行变形梯度凝胶电泳（DGGE）,从DGGE指纹图谱中比较各个结肠癌患者肠道菌群分布情况。结果通过试验得到了维吾尔族异常黑胆质结肠癌肠患者肠道菌群结构特征的DNA指纹图谱,从指纹图谱上选择一些特异性条带切下来回收,测序,测序出来的序列在基因库进行比对做出进化树了解菌群之间的亲缘性。结论异常黑胆质结肠癌患者肠道菌群基因序列的亲缘性结果显示肠道菌群的多样性明显少其中肠道优势菌比例低。

Objective To analyze the traditional uighur medicine abnormal sawda hilit people of colon cancer intestinal flora diversity by advanced molecular biology methods.Methods Choice the colon cancer abnormal sawda hilit by uighur medicine and stool samples were collected from these groups and total genomic DNA was extracted.Extraction of total DNA designed a pair of universal primers to amplify the 16 SrDNA V6to V8 variable regions.Denaturing gradient gel electrophoresis（DGGE）of the amplified sequences was performed then cloned the DGGE bands and sequencing.Results Obtained by experiment reflect structural features of intestinal flora DNA fingerprinting,from sequence specific band sequencing out in the Genebank database on the blast program to compare,and make the evolutionary tree understanding of the relationship between intestinal microflora.Conclusion Colon cancer abnormal sawda hilit intestinal microbial gene sequence showed bacteria have rarefaction state and Dominant bacteria are significantly lower.