耐甲氧西林金黄色葡萄球菌的SCCmec分型及耐药性分析

Methicillin-resistant Staphylococcus aureus SCCmec parting and resistance analysis

目的探讨临床分离的346株耐甲氧西林金黄色葡萄球菌(MRSA)菌株的临床分布、SCCmec分型及其对常用抗菌药物的耐药情况。方法选择2014年1月至2015年1月该院分离的784例金黄色葡萄球菌(SA),采用PCR方法对其进行MRSA及MRSA SCCmec基因分型鉴定,并对其检出分布及耐药性进行分析。结果 784株SA中MRSA 346株(占44.13%),MRSA中来自痰液占43.06%,伤口分泌物占48.55%;MRSA对青霉素、左氧氟沙星、红霉素耐药性较高,对万古霉素、利奈唑胺、替考拉宁均敏感。MRSA经SCCmec分型,共发现SCCmecⅡ型130株,SCCmecⅢ型196株,SCCmecⅣ型11株,SCCmecV型9株。结论该院分离的MRSA以SCCmecⅢ型为主,MRSA对多种抗菌药物具有耐药作用,但对万古霉素、替考拉宁等敏感。

Objective To analyze the clinical distribution,antimicbial resistance and Staphylococcal cassette chromosome mec（SCCmec）genotype characteristics of 346 methicillirrresistant Staphylococcus aureus（MRSA）clinical isolates in the hospital.Methods A total of 784 strains of Staphylococcus aureus isolated from January 2014 to January 2015 in the hospital,MRSA identification and the SCCmec genotype was conducted by PCR assay.Results 346 strains of MRSA（44.13%）were isolated from 784 strains of Staphylococcus aureus,the detection rate of MRSA from sputum accounted for 43.06%,the secretion accounted for48.55%.MRSA was resistant to penicillin,levofloxacin and erythromycin,sensitive to vancomycin,linezolid and teicoplanin.SCCmec genotyping result showed that SCCmecⅡ was identified in 130,SCCmecⅢin 196,SCCmecⅣin 11,SCCmecⅤin 9.Conclusion SCCmecⅢis the main genotypes of MRSA from our Hospital,all of the MRSA strains are multi-resistant to tested antibiotics,but sensitive to vancomycin and teicoplanin.