环丙沙星联合乳铁蛋白多肽嵌合体对铜绿假单胞菌PAO1生物被膜和QS系统信号分子表达的影响

Fit for Pseudomonas Aeruginosa PAO1 Biological Membrane and QS Signal Molecule Expression Effect of Ciprofloxacin Combined with Lactoferrin Polypeptide Chimera

目的:对比分析环丙沙星(CIP)、乳铁蛋白多肽嵌合体(LFchimera)单独使用和二者联合使用处理铜绿假单胞菌对其生物膜及密度感知(QS)系统信号分子(AHL)生成的抑制作用。方法:以铜绿假单胞菌野生菌株PAO1作为实验菌株,随机分为5组,分别为对照组(未做任何处理),实验组分为CIP(0.04μg/ml)处理组、LFchimera(0.25μmol/L)处理组、LFchimera(1.00μmol/L)处理组、CIP(0.04μg/ml)+LFchimera(1.00μmol/L)处理组,分别定量测定且比较各组PAO1生物被膜和QS系统信号分子表达情况。结果:CIP组、LFchimera(0.25μmol/L)组、LFchimera(1.00μmol/L)组、CIP+LFchimera组与对照组相比;LFchimera(1.00μmol/L)组与CIP组/LFchimera(0.25μmol/L)组相比;CIP+LFchimera组与其他所有组相比,POA1生物膜和QS系统信号分子表达均下降,差异有统计学意义(P<0.05)。CIP组与LFchimera(0.25μmol/L)组相比,差异无统计学意义(P>0.05)。结论:CIP和LFchimera联用抑菌作用增强,此抑制作用可能与抑制QS系统信号分子有关。

Objective：To compare and analyze the Ciprofloxacin（CIP） and Lactoferrin Polypeptide Chimera（LFchimera） used alone and combined use of pseudomonas aeruginosa inhibition of biofilm and quorum sensing（QS） system signaling molecules（AHL） were generated.Method：Pseudomonas aeruginosa strain PAO1 was used as the control group（untreated）,CIP（0.04 μg/ml） group,LFchimera（0.25 μmol/L） group,LFchimera（1.00 μmol/L） group,CIP（0.04μg/ml）＋LFchimera（1.00 μmol/L） group,the expression of POA1 and QS system were checked.Result：CIP group,LFchimera（0.25 μmol/L） group,LFchimera（1.00 μmol/L）,CIP＋LFchimera group and control group,LFchimera（1.00 μmol/L） group and CTP group/LFchimera（0.25 μmol/L） group,CIP＋LFchimera group and other groups,the expression of POA1 and QS system were significantly decreased,the differences were significant（P〈0.05）.There was no significant difference between CIP group and LFchimera（0.25 μmol/L） group,the difference was no statistically significant（P〉0.05）.Conclusion：The inhibitory effect of CIP and LFchimera was enhanced,which may be related to the inhibition of QS signaling molecules.