冻融后损伤胚胎发育潜能对临床结局的影响

Impact of developmental potential of injury frozen- thawed embryo on the clinical outcomes

目的探讨冻融后部分损伤胚胎发育潜能对临床结局的影响。方法回顾性分析2013年7月至2014年10月哈尔滨医科大学附属第一医院生殖中心310个出现部分损伤的冻融胚胎移植周期,根据过夜培养后胚胎继续发育情况分为两组,胚胎解冻第二天未继续发育的41个周期为A组,解冻第二天至少有1枚胚胎继续发育的269个周期为B组。比较两组的种植率及临床妊娠率。结果 B组的种植率及妊娠率[17.83%(110/617)、32.34%(87/269)]均高于A组[7.69%(7/91)、14.63%(6/41)](P<0.05)。结论冻融后部分损伤胚胎,过夜培养后未继续发育者种植率及妊娠率低,发育潜能是影响临床结局的重要因素。

Objective To explore the influence of developmental potential of partly injured frozen- thawed embryo on the clinical outcomes. Methods A total of 310 frozen- thawed embryo transfer（ FET） cycles with blastomere damage from July 2013 to October 2014 in reproductive medicine center of first affiliated hospital of Harbin medical university were retrospectively analyzed,all cases were divided into two groups according to further development after overnight cultured,41 cycles whose transferred embryos without development were selected as Group A,269 cycles who had at least one embryo with development were selected as Group B.The clinical pregnancy rate and implantation rate in the two groups were compared. Results The clinical pregnancy rate and implantation rate in the group B[17. 83 %（ 110 /617） 、32. 34 %（ 87 /269） ]were significantly higher than those in the group A[7. 69 %（ 7/91） 、14. 63 %（ 6/41） ]（ P 〈 0. 05）. Conclusion The implantation rate and pregnancy rate of blastomere injury frozen- thawed embryos without development after overnight cultured were low,development ability is one of key factors affecting clinical outcomes.