薄型子宫内膜PI3K、AKT、p-AKT的表达研究

Study on the expression of PI3K,AKT,p-AKT in thin endometrium

目的研究磷脂酰肌醇-3-激酶(PI3K)、蛋白激酶(AKT)、p-AKT在薄型子宫内膜组织中的表达情况,探索PI3K/AKT信号通路在薄型子宫内膜中作用。方法收集2013年8月至2015年1月妇科子宫内膜40份,正常内膜组20份,薄型内膜组20份。采用RT-PCR检测PI3K、AKT mRNA表达,免疫组化S-P法PI3K、AKT、p-AKT蛋白质表达。结果 PI3K及AKT mRNA和蛋白质表达在薄型内膜组明显降低,2组差异有统计学意义(P<0.05)。pAKT蛋白质表达在薄型子宫内膜组明显降低,2组对比差异有统计学意义(P<0.05)。PI3K、AKT及p-AKT蛋白质表达水平呈正相关(r值分别为0.708、0.651、0.650、0.632、0.611、0.623,P<0.05)。结论薄型子宫内膜组织增殖期PI3K、AKT及p-AKT表达下降,其发生及发展可能与PI3K/AKT信号通路有关。

Objective To investigate the expression of PI3K, AKT, p-AKT in thin endometrium tissues, and to explore the effect of PI3K/AKT signal pathway on thin endometrium. Methods Forty endometrium specimens were collected from Department of Gynecology of our hospital from August 2013 to January 2015, including 20 cases of normal endometrium tissues and 20 cases of thin endometrium tissues. The expression levels of PI3K,AKT mRNA were detected by RT-PCR, and the expression levels of PI3K, AKT, p-AKT protein were detected by immunohistochemisty （SP）. Results The expression levels of PI3 K, AKT mRNA and protein, p-AKT protein in thin endometrium group were significantly decreased, as compared with those in normal endometrium group （ P 〈 0.05 ）. There was a positive correlation in the expression levels of PI3K, AKT, p-AKT protein between normal endometrrium and thin endometrrium （ r = 0. 708,0. 651,0. 650,0. 632,0. 611,0. 623, respectively, P 〈 0.05）. Conclusion The expression levels of PI3K, AKT and p-AKT in thin endometrium at proliferation period are significantly decreased, and PI3K/AKT signal pathway may play an important role in the pathogenesis and development of thin endometrrium.