基于RNA-seq识别布鲁菌酸调控候选基因

Identifying Candidate Genes of Acid Regulation Genes of Brucella Based on RNA-seq

布鲁菌作为胞内寄生菌,在胞内生存面临很多压力。特别是感染早期,胞内的酸化是布鲁菌生存的必要条件。本试验利用RNA-seq,对正常培养条件、酸性培养条件的布鲁菌进行比较转录组学分析。结果表明,在酸性条件下113个基因表达发生显著变化(IlogRatiol≥3),其中44%表达上调。经pathway分析,差异基因分布在51个通路,其中与布鲁菌胞内生存及毒力相关的通路有6个,分别是氧化磷酸化、离子转运、细菌分泌系统、转录调节系统、二元转运调控系统和ABC转运系统。布鲁菌对于酸性生存环境表现多通路、多基因的适应性变化,该研究为系统掌握酸调节基因奠定基础。

BruceUa melitensis, a facuhative bacteria, encounters a very stressful environment in phagosomes, especially at low pH levels. In our study, comparative transcriptomes with RNA-seq were used to analyze the changes of genes in normal-medium culture and in prig.g-medium culture. The results revealed that 115 genes expressed with significant differences （Ilog2Ratiol ≥ 3 ）, and about 44% genes expressed as up-regulated. These genes distributed in 51 pathways, in which ribosome and photosynthesis pathways were significantly enriched. Six pathways （oxidative phosphorylation, iron-transporting, bacterial secretion system, tran- scriptional regulation, two-component system, and ABC transporters pathways） tightly related to the intracellular survival and viru- lence of Brucella were analyzed. Global changes involved in many pathways and genes were adapted to the acidic environment of Brucella.