新疆部分地区牛支原体病的血清学调查和病原的分离鉴定

Serological investigation of bovine Mycoplasma disease and isolation and identification of Mycoplasma bovis in some areas of Xinjiang

为了初步了解牛支原体的感染情况,更好地防控牛呼吸道疾病。对新疆部分地区195份牛血清,采用间接ELISA方法,进行了牛支原体的抗体检测;对其鼻拭子进行了病原的分离培养,对培养物进行了形态、生化特性和PCR鉴定。结果显示,牛支原体感染率平均为19.0%,分离到1株牛支原体。在防控牛呼吸道疾病时应考虑牛支原体感染。

In order to study the infection of Mycoplasma bovis and provide better prevention and control of bovine respiratory disease. 195 bovine sera from areas of Xinjiang were analyzed for antibodies against Mycoplasma bovis using indirect ELISA meth- od, The nasal swabs were cultured and the cuhure was identified using morphological, biochemical and PCR methods. The re- sults showed that the average infection rate of Mycoplasma bovis was 19% and one strain of Mycoplasma bovis was isolated. The My- coplasma bovis infection should be considered when preventing and controlling bovine respiratory disease.