人的正常卵巢及卵巢癌绒毛膜促性腺激素受体的研究

COMPARATIVE STUDY ON HUMAN CHORIONIC GONADOTROPIN RECEPTOR IN NORMAL HUMAN OVARY WITH OVARIAN TUMORS

活检正常卵巢标本23例和卵巢癌标本16例(粘液性和浆液性囊腺癌各8例),分离细胞膜作绒毛膜促性腺激素受体结合反应。结果表明:正常卵巢、粘液性及浆液性卵巢癌之平均最大结合率分别为13.30±2.24％、20.15±5.14％及9.06±6.40％;受体量分别为0.66±0.14×10^(-10)mol/μg膜蛋白、1.97±1.24×10^(-10)mol/μg膜蛋白及0.48±0.10×10^(-10)mol/μg膜蛋白,Kd值分别为10.10±5.50×10^(-9)mol、25.00±0.29×10^(-9)mol及16.90±0.14×10^(-9)mol。

Human Chorionic Gonadotropin (HCG)is major physiological luteotropic factors forthe human corpus luteum. The observationsstrongly suggest that the human ovarypossesses a gonadotropin receptor in the cellmembrane. We studied the HCG receptor innormal human ovary and ovarian tumors. Twenty-three human ovarian specimensand 16 ovarian tumor specimens were ob-tained from women patients having gyneco-logical surgery Ovaries were homogenizedand sonicated. The homogenates were cen-trifuged at 2000g for 15 min. After sucrosedensity gradient ultracentrifugation (78000g, 4h) ,two fractions were collected from layerof 33% and interface between 33% and 37%.Thirty μg of ovarian ptotein, 8ng ^(125)I-HCGand unlabeled HCG in a final volume of0.5 ml of 0.05 mol/L Tris buffer were incu-bated at 30℃ for 2h. The results were shown in the table.