摘要
为进一步研究凹唇壁蜂低温下安全越冬技术,通过对凹唇壁蜂体内生理指标检测,初步研究了生理指标变化与抗寒性之间的关系。结果表明:凹唇壁蜂体内的含水量与体内各种酶含量的变化与抗寒性之间没有直接联系,而氨基酸、脂肪、蛋白质和甘油随着温度的逐渐降低,有逐渐增加并积累的趋势,在凹唇壁蜂的越冬过程中起促进作用,是重要的抗寒物质。同时在人工模拟条件下计算凹唇壁蜂的过冷却点和体液冰点,温度分别为雄性-24.46和-19.41℃,雌性-25.02℃和-19.97℃。本地的最低极端温度能达到-30℃以下,说明凹唇壁蜂在室外不能安全越冬。采取保护措施可以使凹唇壁蜂在本地安全越冬,安全温度应该是-15^-10℃,而决不能低于-18℃。
In order to research the safe wintering technology at low temperature of Osmia excovata Alfken,through the excovata in vivo physiological index detection,apreliminary study of the relationship between physiological changes and cold tolerance was analyzed.The results showed that the change of moisture content and different enzyme content did not have a direct link with the cold resistance.And amino acids,fats,proteins and glycerine with decreasing temperature,there was an increasing trend and gradually accumulated which played a catalytic role in the excovata wintering process,it was an important cold substances.Meanwhile in artificial conditions,the Osmia excavata supercooling points（SCP）and humor freezing point（HFP）were determinated,that indicated that the winter minimum super-cooling point and humor freezing point of male Osmia excavata were-24.46and-19.41℃,-25.02and-19.97℃for female Osmia excavata.As the lowest temperature of the local areas-30℃,Osmia excavatacould not survive safely the winter.Taking protective measures could make Osmia excovatain the local security,safety temperature should be between-15～-10 ℃,and must not be lower than-18℃.
出处
《黑龙江农业科学》
2016年第2期72-76,共5页
Heilongjiang Agricultural Sciences
基金
黑龙江省农业科技创新工程资助项目(2014ZD009)
现代农业产业技术体系建设专项资金资助项目(CARS-28)
