三种多环芳烃混合溶液二维荧光相关谱解析

Analysis of Three Polycyclic Aromatic Hydrocarbons in Solution Based on Two-Dimensional Fluorescence Correlation Spectroscopy

多环芳烃为优先控制污染物,但是由于其含量很低,多组分多环芳烃荧光峰相互重叠,所以常规荧光光谱法无法对其荧光峰进行有效解析。采用二维荧光相关分析方法对三种多环芳烃,蒽、菲和芘的混合溶液进行荧光峰解析。根据研究目标,按照三种多环芳烃浓度比的不同配制了三种混合物体系,共27个样本,每种体系的三种溶液浓度彼此间按规律递增和递减。在此基础上,以浓度为外扰,构建了各体系的同步和异步二维荧光相关谱。同步谱中,在425,402,381,373,365,393及347nm处产生自相关峰。以未被覆盖的菲在347nm处荧光峰为线索,通过其与各波长处荧光交叉峰的正负,判断出了402,381,425和452nm处荧光峰源于混合溶液中的蒽;373与393nm处荧光峰源于混合溶液中的芘;365,356及347nm处荧光峰源于混合溶液中的菲。通过异步谱解析出菲的385nm处荧光峰,证明了异步谱比同步谱具有更好的光谱分辨率。研究结果表明,采用二维荧光相关方法对光谱严重重叠的多组分多环芳烃的解析是可行的,并具有一定的优势,可推广到对环境中其他污染物质的检测。

Polycyclic aromatic hydrocarbons（PAHs）are listed as the priority pollutants.It is difficult to resolve effectively the peaks of PAHs by conventional one-dimensional fluorescence spectroscopy due to its low content and the overlapping fluorescence peaks.In this paper,the two-dimensional（2D）fluorescence correlation spectroscopy will be applied to the analysis of highly overlapped fluorescence spectra of the mixed solution of anthracene,phenanthrene and pyrene.According to the research goals,three mixed systems and a total of 27 samples,are to be prepared with different concentrations of three PAHs.Concentrations of three PAHS are monotonically increasing or decreasing in each mixed system.Then the 2Dfluorescence correlation spectrum of each mixed systems will be calculated under the perturbation of the concentration of anthracene,phenanthrene and pyrene in solution.There are seven strong autopeaks at 425,402,381,373,365,393 and 347nm in synchronous 2Dcorrelation spectrum.The fluorescence peak of phenanthrene at 347 nm is uncovered in three mixed systems,so the band at 347 nm is to be used as clues for further assignment.According to positive or negative cross peaks at 347 nm in synchronous 2Dcorrelation spectrum,we can know that the peaks at 402,381,425 and 452nm are assigned to anthracene,the peaks at 373 and 393nm are assigned to pyrene,and the peaks at 365,356 and 347nm are assigned to phenanthrene.The fluorescence peak of phenanthrene at 385 nm is shown in asynchronous 2Dcorrelation spectrum;it means the spectral resolution of asynchronous spectrum is better than the synchronous spectrum.The results are that it is feasible to analyze serious overlapping multi-component PAHs using two-dimensional fluorescence correlation spectroscopy,which can be extended to the detection of other pollutants in the air.