年轻妇女宫颈鳞状上皮内病变、宫颈癌组织中P16^(InK4a)和Ki-67的表达及其预测作用

The expressions and predictive function of P16^(InK4a) and ki-67 in cervical tissues of young women with cervical squamous intraepithelial lesions or cervical carcinoma

目的:探讨P16^(InK4a)蛋白及Ki-67在年轻妇女宫颈鳞状上皮内病变(CSIL)、宫颈癌组织中的表达及其与不同病变级别的相关性,预测CSIL转归作用。方法 :选择2012年1月~2014年12月在广东省妇幼保健院就诊,年龄<35岁、经病理确诊的宫颈低级别鳞状上皮内病变(LSIL)、宫颈高级别鳞状上皮内病变(HSIL)、宫颈癌患者111例,采用免疫组织化学染色,检测宫颈组织中P16^(InK4a)及Ki-67的表达。结果:1不同级别CSIL及宫颈癌病变组织中,P16^(InK4a)及Ki-67的阳性表达强度与病变级别相关(P<0.001);2P16^(InK4a)在LSIL、HSIL及宫颈癌的阳性表达率分别为24%、81%、95%,Ki-67阳性表达率分别为37%,87%,95%,P16^(InK4a)及Ki-67在不同级别CSIL、宫颈癌的总阳性率有统计学差别(P<0.001)。结论:P16^(InK4a)及Ki-67在年轻妇女CSIL、宫颈癌组织中阳性表达强度与病变级别相关,可辅助临床评价肿瘤生物学行为及预测预后。

Objective： To study the expressions and predictive prognosis of P16^InK4a or ki-67 in cervical tissues of women with cervical squamous intraepithelial lesions and cervical carcinoma, and to analyze the association between the expression of P16^InK4a or ki-67 and different grade of cervical diseases. Methods.. From January 2012 to December 2014, 111 women（〈35 years）with cervical low-grade squamous intraepithelial lesions, high-grade squamous intraepithelial lesions or cervical carcinoma in the Women and Children Health Care Hospital of Guangdong Province were included. All cervical tissues of the included women were performed immunohistochemical staining to detect expressions of P16^InK4a and ki-67.Results： ① The expression of P16^InK4a or ki-67 were related to different grade of cervical diseases （P〈0 0.001）.②In the cervical issue of women with low-grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion or cervical carcinoma, total positive expression rate of P16^InK4a was 24%, 81% and 95%, respectively. And total positive expression rate of ki-67 were 37% ,87% and 95%, respectively. The differences were statistically significant （P〈0.001）. Conclusions： The positive expression of P16^InK4a or ki-67 in cervical issue of young women was correlated with different grade of cervical diseases.The expression of P16^InK4a and ki-67 can assist to evaluation tumor biological behavior in clinical medicine and to predict prognosis of cervical diseases.