食品包装材料中丙烯酰胺在大鼠肝微粒和S9中的体外代谢研究

Metabolism of acrylamide by rat liver microsomes and S9 in vitro

目的研究食品包装材料中丙烯酰胺(acrylamide)的体外代谢情况,并确证其代谢产物。方法采用肝微粒体及肝S9体外温孵法,优化代谢条件,对丙烯酰胺进行了体外代谢研究,并用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)检测并确证丙烯酰胺的代谢物。结果通过检测结果发现,丙烯酰胺分别在肝S9和肝微粒体作用下发生显著的代谢反应,其代谢产物之一为环氧丙酰胺。结论丙烯酰胺能通过代谢转化成毒性更强的环氧丙酰胺,因此,监控食品、饮水和周围环境中的丙烯酰胺的含量对于维护人们的健康具有重要的意义。

Objective To investigate the metabolism and the metabolin of acrylamide in rat liver microsomes and $9 in vitro. Methods The metabolism was researched in rat liver microsomal and S9 incubation systems in vitro. Liquid chromatography-tandem mass spectrometry （LC-MS/MS） method was developed to determine acrylamide and its metabolin. Results Acrylamide was evidently metabolized by rat liver microsomes and S9, and one of the metabolins was found to be glycidamide. Conclusion Acrylamide can be metabolized to more toxic compound, glycidamide. Therefore, it is of great significance to monitor acrylamide in food, drinking water and the surrounding environment, for the maintenance of people＇s health.