食品包装材料中有害物质双酚A二缩水甘油醚体外代谢研究

In vitro metabolic study of bisphenol A diglycidyl ether in food contact materials

目的通过模拟体内代谢,对双酚A二缩水甘油醚(BADGE)的体外基本代谢情况进行研究。方法采用肝微粒体、肝S9 2种体外代谢试剂,通过模拟体内肝脏代谢,对BADGE的代谢行为及其代谢产物进行研究。通过代谢试剂浓度及代谢时间条件的优化,采用高效液相色谱-串联质谱(HPLC-MS/MS)作为检测手段对BADGE的体外代谢产物进行分析确证。结果体外代谢最佳孵化时间为60 min,最佳体外代谢试剂浓度为0.5mg/m L,在肝S9及肝微粒体2种体外代谢试剂的作用下,BADGE发生显著的代谢反应。结论本研究与传统的动物试验相比,节约了时间、精力,对食品包装材料的毒理学研究和安全性评价有重要的推动作用。

Objective To investigate the in vitro metabolism ofbisphenol A diglycidyl ether （BADGE） by simulating in vivo metabolic conditions. Methods The conditions of in vitro metabolic reaction including the concentration of liver microsome and S9 and reaction time were optimized. The metabolic products of BADGE were analyzed and verified by high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）. Results The best incubation time was 60 rain, and the most suitable reagent concentration was 0.5 mg/mL. BADGE was converted significantly under the action of in vitro metabolism reagent of liver S9 and microsome. Conclusion The study is simple and has the advantages of shorter reaction time and energy compared with the traditional animal experiments, and it has an important role in promoting the toxicology research and safety evaluation of food packaging materials.