荧光增白剂致中国仓鼠卵巢细胞微核形成与基因突变研究

Effect of fluorescent brightener on micronucleus and gene mutation of Chinese hamster ovary cells

目的研究荧光增白剂诱导中国仓鼠卵巢细胞微核形成和基因突变的作用,探讨荧光增白剂对体外哺乳动物细胞的遗传物质损伤机制。方法使用不同浓度的荧光增白剂28处理中国仓鼠卵巢细胞,通过体外哺乳动物细胞基因突变实验和体外哺乳动物细胞微核实验检测细胞遗传物质的损伤程度。结果 0.5556 mg/m L组和1.6667 mg/m L组hprt基因突变频率和微核率与阴性对照组无显著性差异,5.000 mg/m L组hprt基因突变频率和微核率显著高于阴性对照组(P<0.05),阳性对照组hprt基因突变频率和微核率显著高于阴性对照组(P<0.01)。结论荧光增白剂28可引起体外细胞的遗传物质损伤。

Objective To study the effect of fluorescent brightener on micronucleus and gene mutation of Chinese hamster ovary cells （CHO）, and to discuss the mechanism of genetic damage of mammalian cell in vitro. Methods The genetic damage of CHO cells treated with different concentrations of fluorescent brightener 28 was detected with the method of mammalian cell micronucleus test and mammalian cell gene mutation test in vitro. Results There was no significant difference of hprt gene mutation frequencies and micronucleus rates between negative control group and 0.5556 mg/mL and 1.6667 mg/mL fluorescent brightener 28 treated groups. The hprt gene mutation frequency and micronucleus rate of CHO cells with 5.000 mg/mL fluorescent brightener 28 were significant higher than that of negative control group （P〈0.05）. The hprt gene mutation frequency and micronucleus rate of CHO cells in positive control group were significant higher than that of negative control group （P〈0.01）. Conclusion Fluorescent brightener 28 may induce genetic damage in cultured mammalian cells.