酪蛋白水解物与γ-氨基丁酸复配制剂改善睡眠功能的研究

Study on improvement of sleep function by the compound of casein hydrolysate and γ-aminobutyric acid

目的研究酪蛋白水解物与γ-氨基丁酸的复配制剂对小鼠改善睡眠功能的作用研究。方法 18~22 g健康雄性小鼠,按体重随机分组。按照人体推荐剂量的5倍、10倍、30倍为低、中、高3个剂量,即0.1、0.2和0.6 g/kg bw,另设对照组。观察睡眠指标。结果 3个剂量组入睡动物数及睡眠时间均为0,各剂量组动物入睡数与对照组进行比较,差异均无统计学意义（P〉0.05）。该制剂与对照组比较可延长中、高剂量组小鼠戊巴比妥钠催眠的时间（P〈0.05）,可缩短巴比妥钠催眠的中、高剂量组小鼠的入睡潜伏期,与对照组比较差异有统计学意义（P〈0.05）。结论根据《保健食品检验与评价技术规范》对改善睡眠保健食品的判定依据,酪蛋白水解物与γ-氨基丁酸的复配制剂具有一定的改善睡眠作用。

Objective To study the improvement of the sleep function by the compound of casein hydrolysate and ）,-aminobutyric acid on mice. Methods Healthy male mice with the body weight of 18-22g were divided into different groups randomly. According to the recommended dosage of 5 times, 10 times, 30 times, the mice were divided into low（0.1 g/kg bw）, medium （0.2 g/kg bw）and high（0.6 g/kg bw） dose groups, and another control group was set, to observe the sleeping indexes of mice. Results The number of sleeping animals and sleep time of the 3 groups were 0, and the difference was not statistically significant （F〉0.05）compared with the control group. Compared with the control group, the compound preparation of casein hydrolysate and γ-aminobutyric acid could prolonged the time of the sodiumin mice with middle and high dose group（P〈0.05） and shorten the latency of sleep in the middle and high dose group of mice （P〈0.05）.Conelusion According to the judgment basis on improving the sleep health from ＂Technical specification for inspection and evaluation of health food＂, the compound preparation of casein hydrolysate and γ-aminobutyric acid can improve the sleep quality of mice.