超声提取-离子色谱法检测市售常见15种蔬菜中7种单糖和双糖

Ultrasonic extraction-ion chromatography detection of 7 kinds of monosaccharides and disaccharides in 15 kinds of common vegetables

目的建立离子色谱-脉冲积分安培检测法（IC-PAD）测定蔬菜中的水溶性的半乳糖、葡萄糖、木糖、果糖、蔗糖、乳糖和麦芽糖方法。方法采用水浸提超声提取及膜过滤法处理蔬菜样品,以DIONEX Carbo Pac PA10阴离子交换柱为分析柱,以DIONEX Carbo Pac PA10为保护柱,以Na OH与CH3COONa的组合溶液作为流动相,采用梯度淋洗程序分离,脉冲积分安培检测。结果 7种糖的检出限分别为3、4、3、6、6、4和3μg/L,且均具有较宽的线性范围（0.05~20 mg/L）。15种蔬菜样品测定的相对标准偏差在1.48%~6.41%之间,7种糖的加标回收率在90%~97%之间。结论本方法检测糖简便快捷、分离效果好、无需衍生、灵敏度高,适用于蔬菜中的常见糖组分的分析。

Objective To establish a method for the detection of water-soluble galactose, glucose, xylose, glucose, sucrose, lactose, and maltose in vegetables by ion chromatography - pulse integral amp test （IC-PAD）. Methods Water immersion ultrasonic extraction and membrane filtration method were used to treat vegetable samples, DIONEX CarboPac PA10 anion exchange column as the analysis of column, DIONEX CarboPac PAl 0 as protection column, with the combination of NaOH and CH3COONa solution as mobile phase. Samples were separated by gradient elution program and detected by pulse integral ampere. Results Detection limits of 7 kinds of sugars were 3, 4, 3, 6, 6, 4, and 3μg/L, and all of them had wide linear range （0.05-20 mg/L）. The relative standard deviation of 15 kinds of vegetables was between 1.48% and 6.41%, and the recovery rates of 7 kinds of sugars were between 90% and 97%. Conclusion The established method is easy, effective, sensitive, and does not need to be derivative. It is suitable for the analysis of common sugars in vegetables.