雌、孕激素受体及基质金属蛋白酶9与女性患者痔病严重程度的相关性

Correlation of estrogen receptor,progesterone receptor and MMP-9 expression with hemorrhoidal disease severity in female patients

目的研究雌、孕激素受体及基质金属蛋白酶9(MMP-9)与女性患者痔病严重程度的相关性。方法接受痔切除术的138例女性内痔患者为研究对象,分为Ⅲ度组73例和Ⅳ度组65例。观察年龄、孕次、生产方式(阴道分娩和剖宫产)、是否绝经等情况,切除的标本送病理检测其雌激素受体(ER)和孕激素受体(PR)的表达,比较两组阳性率。采用半定量RT-PCR检测MMP-9 mRNA的表达。结果 Ⅲ度组和Ⅳ度组患者在平均年龄、是否绝经方面,差异无统计学意义(P>0.05)。而在孕次及生产方式方面比较,差异有统计学意义(P<0.05)。两组ER阳性率分别为54.8%和72.3%,PR阳性率分别为52.1%和69.2%,Ⅳ度组MMP-9 mRNA的表达明显高于Ⅲ度组[(0.6858±0.1887 vs 0.5971±0.1860),P<0.01]。结论女性孕次、分娩方式及痔组织中ER、PR及MMP-9水平与患者痔病严重度存在一定的相关性,对痔病的发生发展起到一定作用。

Objective To study the correlation of estrogen receptor （ER）, progesterone receptor and MMP-9 expression with hemorrhoids disease severity in female patients. Methods A total of 138 fe- male patients with internal hemorrhoids accepted haemorrhoidectomy,including 73 cases in grade m group and 65 cases in grade IV group. Age, pregnant time, parturition, and menopausal status were observed. Resected samples were tested for pathological detection of estrogen receptor （ER） and progesterone recep- tor （PR） expressions and comparison. The expression of MMP-9 mRNA were detected by reverse tran- scription polymerase chain reaction （RT-PCR）. Results There were no significant differences in age and menopausal status between groups. There were significant differences in pregnant time and parturition be- tween groups. The positive rates of ER were 54.8% and 72.3% in grade Ⅲ and grade Ⅳ group, respectively. The positive rates of PR were 52.1% and 69.2% ,respectively. Compared with grade m group, the expression of MMP-9 mRNA was obviously increased in the grade Ⅳ group [ （0. 6858 ± 0. 1887 ） vs （0. 5971 ±0. 1860） ,P 〈0.01 ]. Conclusion Pregnant time, parturition and expressions of ER, PR and MMP-9 in hemorrhoid tissues have certain relevance with hemorrhoidal disease severity, and they may play roles in the occurrence of hemorrhoidal disease.