脐血中Caspase7蛋白在羊水量异常产妇中的表达

Expression of Caspase7 in umbilical serum of pregnant women with abnormal amniotic fluid

目的探讨Caspase7蛋白在羊水量异常和正常的产妇血清中的表达差异。方法采用酶联免疫吸附法(ELISA)分别检测30例羊水过少、30例羊水过多和30例羊水量正常产妇脐带血中Caspase7蛋白表达水平的情况,并加以分析。结果Caspase7蛋白在3组产妇血清中均有阳性表达。羊水过少组血清中Caspase7蛋白平均浓度(160.2±25.8ng/mL)比对照组高(142.9±18.4ng/mL),差异有统计学意义(t=2.99,P<0.05)。羊水过多组血清中Caspase7蛋白平均浓度(119.2±21.5ng/mL)比对照组低(142.9±18.4ng/mL),差异也有统计学意义(t=4.59,P<0.05)。结论羊水过少或过多产妇血清中Caspase7蛋白表达异常,提示其可能与羊水量异常的发生有关。

Objective To explore the difference in expression of Caspase 7 in umbilical serum between pregnant women with abnormal amniotic fluid and normal pregnant women .Methods ELISA was performed to determine the concentration of Caspase 7 protein in umbilical serum of 30 cases of oligohydramnios , 30 cases of polyhydramnios and 30 normal cases .Results Caspase7 was positively expressed in umbilical serum of three groups.The expression of Caspase7 was higher in the oligohydramnios group （160.2 ±25.8 ng/mL） than in the normal group （142.9 ±18.4 ng/mL）, and there was significant difference （t=2.99,P〈0.05）.The expression of Caspase7 was lower in the polyhydramnios group （119.2 ±21.5 ng/mL） than in the normal group （142.9 ±18.4 ng/mL） with significant difference （t=4.59, P〈0.05 ） .Conclusion The expression of Caspase 7 in umbilical serum of pregnant women of oligohydramnios and polyhydramnios is abnormal, which indicates that it might be correlated with abnormal amniotic fluid .