茴香提取液对糖尿病大鼠血清NO、MDA和SOD的影响

Effects of Foenicullum vulgare extract on serum nitric oxygen,malondialdehyde and superoxide dismutase in diabetic rats

目的观察茴香提取液干预治疗糖尿病大鼠后血清NO、MDA和SOD的变化,探讨茴香提取液对糖尿病大鼠氧化应激作用的影响。方法采用链脲佐菌素(STZ)诱导制备40只糖尿病大鼠模型,造模后分成模型组、二甲双胍组、茴香低剂量组、茴香高剂量组,每组8只,分别给予蒸馏水、二甲双胍(0.15g·kg^(-1))、茴香提取液(1.5g·kg^(-1))、茴香提取液(3.0g·kg^(-1))灌胃治疗30d,另设正常对照组(n=8)。治疗结束后,取血清检测NO含量、MDA含量和SOD活性。结果与对照组相比,模型组的NO和MDA含量增加,SOD活性下降。与模型组相比,茴香低剂量组和茴香高剂量组的NO、MDA含量下降,SOD活性上升。结论茴香提取液能够降低糖尿病大鼠氧化应激的水平,减轻氧化应激损伤,有利于改善胰岛素抵抗。

Objective The study was designed to observe the changes of nitric oxygen （NO）, malondial-dehyde （MDA） and superoxide dismutase （SOD） in diabetic rats after being administered with Foenicullurn vulgate extract and to investigate the effects of Foenicullurn vulgate （F. vulgar） extract on oxidative stress of diabetic rats. Methods The diabetic rat models were induced by streptozotocin （STZ）. After the models were established, the diabetic rats were randomly divided into a model group, a metformin group, a low-dose F. vulgare group and a high-dose F. vulgare group, eight diabetic rats in each group. The distilled water,metformin 0.15 g· kg-1 , F. vulgare 1.5 g · kg-1 , F. vulgare 3.0 g · kg-1 perfused the stomach in the corre- sponding group for 30 days. And 8 rats were chosen as normal group. After the last dose, NO contents, MDA contents and SOD activity in all rats serum were measured. Results Comparing the controt group with the model group, NO and MDA contents in the model group were increased and SOD activity was decreased. Compared with the model group, SOD activity was increased in high and low-dose F. vulgare groups, the MDA contents and NO contents were decreased. Conclusion F. vulgar treatment can downregulate oxidative stress in diabetic rats, relieve oxidative stress injury and benefit for improving insulin resistance.