荧光法测定悬浮培养藻体叶绿素含量的研究

Study about Chlorophyll Fluorescence Determination with the Aalgae Suspension Culture

实验室常采用荧光检测技术实现对藻的定量检测。藻细胞沉降，导致其荧光值受静置时间影响较大，静置1min时，荧光值的衰减率达△F／F0=-9．96％，无法获得准确的藻浓度。因此，本文提出一种藻体样品制备方法，培养过程中添加琼脂粉（0．08-0．10g／100mL）作为藻液悬浮稳定剂。用该方法培养的藻液其悬浮性、稳定性均有明显提高，且静置1h后，△F／F0=-4．82％≤±5％。减少了藻体沉降对测定结果的影响，确保了荧光光度法测定叶绿素含量的准确性。

Laboratory always obtain the content of algae volume by measuring the chlorophyll a fluorescence intensity. According to the settlement of algae ceils, fluorescence value are greatly influenced by static duration, when the static duration is lmin, attenuation rate of fluorescence was △F/Fo = -9. 96% and the determination of algae density is not exact. In the article, a method of algae culture preparation was established by adding agar powder（ 0. 08- 0. 10g/100mL） to the culture. The suspensibility and stability of the culture was enhanced dramatically by this method, after standing 1 hour, attenuation rate of fluorescence was △F/Fo =-4. 82% ≤ ±5%. The method enhance the accuracy of chlorophyll contents measurement by chlorophyll spectrofluorimetry and reduces the effect of culture sedimentation to the measurement.