荧光标识生物分子检测系统研究及性能分析

Detection System of Fluorescently Labeled Biological Molecules and Its Performance Analysis

为了确定待测样品中是否存在某种特定的基因、蛋白质,设计了一种能够对荧光探针标识的生物分子进行检测的荧光偏振系统.本文分析了分光光度法、共振光散射分析法所存在的问题和荧光偏振检测的优势和特点,研究了荧光偏振检测系统的原理,设计了检测光路和测控系统,并进行了大量的生物检测实验,实验结果表明该检测系统能够确定待测样品中是否存在需要检测的物质,其测试的重复性误差小于3%,正确率可达到100%,孔位测试不均匀性小于2%.

In order to determine whether there is specific gene or protein in the sample,a fluorescent polarization system for detecting biomolecules was designed. This paper analyzes the disadvantages of the spectrophotometry and resonance light scattering analytical methods, describes the advantages and characteristics of the fluorescence polarization detection, studies the principle of fluorescence polarization detection system, and designs the optical path and the control system. A large number of biological testings were conducted with the new system. Experimental results show that the detection system is able to determine whether there is suhstance to be detected in the sample. The repeatability error is less than 3% ,the accuracy rate is up to 100% and the unevenness of the holes test is less than 2%.