异养硝化苯酚降解菌的筛选及性能研究

Screening of heterotrophic nitrifying phenol-degrading strain and its characteristics

从武汉市某石油化工厂的活性污泥中筛选出1株能降解苯酚的异养硝化菌Y10,通过生理生化试验及16S r DNA测序鉴定其为Pseudomonas sp.属细菌。该菌生长和降解苯酚的适宜条件为:温度为35℃,培养基初始p H为9.0,摇床转速为200 r/min。在该条件下,质量浓度为837 mg/L及1 254 mg/L的苯酚分别在7 h和20 h时被完全降解,降解速率分别为119.6 mg/(L·h)和62.7 mg/(L·h)。以苯酚为唯一碳源且碳源充足时,Y10能将初始质量浓度为114 mg/L的氨氮在12 h时全部降解,该过程产生的亚硝酸盐氮≤1.5 mg/L,表明该菌可实现同步硝化反硝化。

A phenol heterotrophic nitrifier Y10 is isolated from the activated sludge of a petrochemical refinery. It is identified as a member of the genus Pseudomonas sp. according to its morphological and physiological properties and the analysis of its 16S rDNA gene sequence. The optimal conditions for its growth and phenol removing are as follows ：35℃ of temperature ,9. 0 of initial pH of growth medium and 200 r/min of shaking speed. Under this condition, the phenol can be completely decomposed at 7 hours and 12 hours with the corresponding degradation rate of 119.6 mg/（ L＂ h） and 62. 7 mg,/（L.h）, when phenol concentration are 837 mg/L and 1254 mg/L, respectively. When phenol is used as the sole carbon source,this strain can degrade 114 mg/L of ammonia nitrogen completely within 12 hours. In this process, the production of nitrite nitrogen was below 1.5 mg/L. The results show that the strain can achieve simultaneous nitrification and denitrifieation.