摘要
目的探讨晚期非小细胞肺癌患者外周血CD4^+ CD25^+ FOXP3^+ 调节性T(Treg)细胞的表达及其临床意义。方法采用免疫荧光术及流式细胞仪检测50例晚期非小细胞肺癌患者及50例健康对照组外周血中CD4^+ CD25^+ FOXP3^+ Treg细胞、CD4^+ CD25^+ Treg细胞、CD4^+ T细胞和CD4^+ CTLA-4^+ T细胞的表达。结果晚期非小细胞肺癌患者外周血中CD4^+ CD25^+ FOXP3^+ Treg细胞、CD4^+ CD25^+ Treg细胞和CD4^+ CTLA-4^+ T细胞的比例均高于健康对照组(均P<0.05),CD4^+ T细胞的比例均低于健康对照组(均P<0.05)。结论晚期非小细胞肺癌患者外周血CD4^+ CD25^+ FOXP3^+ Treg细胞比例高于健康对照者,可能与肺癌患者的免疫抑制和肿瘤进展相关。
Objective To demonstrate the possible involvement of CD4＋CD25 ＋FOXP3 ＋ regulatory T （Treg） cells in immune system impairment in patients with advanced non-small cell lung cancer. Meth- ods The CD4 ＋ CD25 ＋ FOXP3 ＋ Treg cells, CD4＋CD25 ＋ Treg cells, CD4＋ T cells and CD4＋ CTLA-4 ＋ T cells were analyzed in peripheral blood in 50 patients with advanced non-small cell lung cancer and 50 healthy controls. Results Compared with healthy controls, patients with advanced non-small cell lung cancer had higher percentage of CD4 ＋ CD25 ＋ FOXP3＋ Treg cells, CD4 ＋ CD25 ＋ Treg cells and CD4 ＋ CTLA- 4 ＋T cells, had lower percentage of CD4＋ T cells （ P 〈 0. 05 ）. Conclusion The increased percent of CD4＋ CD25 ＋ FOXP3＋ Treg cells may be related to immunosuppression and tumor progression in patients with advanced non-small cell lung cancer.
出处
《中国肿瘤临床与康复》
2016年第2期134-137,共4页
Chinese Journal of Clinical Oncology and Rehabilitation
