黑龙江地产山里红叶醇提液对急性心肌缺血大鼠血流动力学和血小板凝聚的影响

Effects of Ethanol Extract of Crataegus Pinnatifida Bge. Var. major N.E.Br. from Heilongjiang Province on Hemodynamics of Acute Myocardial Ischemia and Platelet Aggregation in Rats

目的:观察黑龙江省地产山里红叶醇提液对大鼠结扎造模后引起心肌缺血的血流动力学变化及注射异丙肾上腺素引起急性心肌缺血血小板凝聚的影响。方法:结扎造模建立大鼠急性心肌缺血大鼠模型,分别测定动脉收缩压(SBP)、动脉舒张压(DBP)、心率(HR)、左室收缩压(LVSP)、左室舒张末压(LVEDP)、左室内压最大上升(+)和下降(-)速率(±dp·dt max^(-1))。以异丙肾上腺素建立大鼠急性心肌缺血模型,用血小板聚集仪测定血小板聚集率。结果:黑龙江地产的山里红叶醇提液高剂量组可显著升高SBP、DBP、LVSP、+dp·dtmax^(-1)和HR,降低LVEDP,-dp·dtmax^(-1),差异均有统计学意义(P<0.05)。中剂量组除了对DBP影响较弱外,其余指标差异均有统计学意义(P<0.05);低剂量组仅能对SBP、LVSP、±dp·dtmax^(-1)起到明显作用,差异均有统计学意义(P<0.05),其余则效果不明显。在测定1min,5min和最大的聚集率时,山里红叶醇提液高、中剂量组均显著抑制血小板凝聚,差异均有统计学意义(P<0.05)。结论:黑龙江地产山里红叶醇提液可改善急性心肌缺血大鼠的血流,减少缺血后血小板凝聚的作用。

Objective： To observe the effect of ethanol extract of Crataegus Pinnatifida Bge. Vat. major N. E. Br. from Heilongjiang province on hemodynamics of acute myocardial ischemia caused by ligation and platelet aggregation induced by isoproterenol. Methods： After the model establishment of acute myocardial ischemia by ligation in rats, the systolic blood pressure （SBP）, diastolic blood pressure （DBP） , heart rate （ HR）, left ventricular systolic pressure （ LVSP）, left ventricular end-diastolic pressure （ LV- EDP）, left ventricular peak systolic （ ＋ ） and diastolic （ - ） rate （ ＋ dp · dt max -1 ） were recorded. Meanwhile, the acute myocardial ischemia model induced by isoproterenol was established. The platelet aggregation was measured by a platelet aggregation instrument. Results： The ethanol extract of Crataegus Pinnatifida Bge. Var. major N. E. Br. from Heilongjiang province could significantly increase the SBP, DBP, LVSP, ＋dp · dtmax -1 and HR, and reduce LVEDP,-dp · dtmax- 1 in high dose group with statistical significance （P 〈0.05）. The medium dose group had small impact on the DBP, while showed statistically significant effect on the other hemodynamic indices. The low dose group had statistically significant effect on the SBP, LVSP and ± dp -1 dtmax-1 （ P 〈 0.05 ）, while showed no notable effects on the other indices. The results of aggregation rate in 1 rain and 5 min after the determination and the maximum one in the high and medium dose groups showed that the ethanol extract could significantly inhibit the platelet aggregation with statistical significance （ P 〈 0.05 ）. Conclusion： The ethanol extract of Crataegus Pinnatifida Bge. Var. major N. E. Br. can improve the blood flow of acute myocardial ischemia in rats and reduce platelet aggregation after ischemia.