HPLC测定百草妇炎清栓中蛇床子素的含量

Determination of Osthole in Baicao Fuyanqing Suppositories by HPLC

目的:建立测定百草妇炎清栓中蛇床子素含量的高效液相色谱方法。方法:以日本DL-C_(l8)柱(4.6 mm×150 mm,5μm)为色谱柱,乙腈-水(65∶35)为流动相,检测波长321 nm,流速为1.0 ml·min^(-1)。结果:蛇床子素在浓度为2.0~80.0g·ml^(-1)范围内有良好的线性关系,相关系数为0.999 1,平均加样回收率为97.5%,RSD为1.95%(n=6)。结论:本方法操作简单、快速、准确,可用于百草妇炎清栓中蛇床子素的含量测定。

Objective： To establish a method for determining osthole in Baicao Fuyanqing suppositories by HPLC. Methods： The determination was carried out on a DL-C18 column （4.6 mm × 150 mm, 5 μm）. The mobile phase consisted of acetonitrile-water （65：35） with the detection wavelength at 321nm and the flow rate of 1.00 ml · min-1. Results： The linear range of osthole was 2.0-80.0 μg · ml-1 （ r =0.999 1 ）. The average recovery of osthole was 97, 5% （RSD = 1.95% ,n =6）. Conclusion： The method is simple, rapid and accurate, which can be used for the determination of osthole in Baicao Fuyanqing suppositories.