蓝狐胆汁对D-Galn肝损伤小鼠模型保护作用的研究

Study on the Protection of Blue Fox Bile on Liver-injury Model Induced by D-Galn in Mice

目的:探讨蓝狐胆汁对D-Glan肝损伤小鼠模型的保护作用。方法:取昆明种小鼠30只,随机分为5组,即空白对照组(空白组)、D-氨基半乳糖(D-Galn)急性肝损伤模型组(造模组)、阳性药葵花护肝(0.4 g/kg)组(阳性组)、蓝狐胆粉高剂量组(10 g/kg)组(高剂量组),蓝狐胆粉低剂量(5 g/kg)组(低剂量组),每组6只;灌胃给药,1次/d,连续10 d,空白对照组予同体积蒸馏水。末次给药前24 h,小鼠腹腔注射D-Galn 500 mg/kg,末次给药前15 h将动物禁食,末次给药后1 h将动物处死,取血清,测定血清中总胆固醇(T-CHO)浓度、谷丙转氨酶(ALT)和谷草转氨酶(AST)的活性;取肝脏,测定肝脏中的丙二醛(MDA)含量,并作病理切片。结果:与模型组比较,蓝狐胆粉高剂量、低剂量组均能显著降低血清ALT、AST、T-CHO及肝脏MDA的含量(P<0.05),表明蓝狐胆汁具有保护D-Galn造成肝损伤的作用。结论:蓝狐胆汁对D-Galn造成肝损伤小鼠模型具有保护作用。

Objective： To investigate the protective effect of blue fox bile on D-Glan liver injury model in mice. Methods： The 30 Kunming mice were randomly divided to five groups： blank control group （blank group）, D-galactosamine （D-Gain） acute liver injury model group （model group）, positive drug sunflower protects liver （0.4 g/kg） group （schizophrenia）, blue fox bile powder in high dose group （10 g/kg） group （high dose group）, blue fox bile powder of low dose （5 g/kg） group （low dose group）, with 6 rats in each group. Intragastric administra- tion, once daily, continuous 10 days and blank control group was given the same volume of distilled water. End time for medicine before 24 h, mice were injected intraperitoneally with D-Gain 500 mg/kg, the last before ad- ministration of 15 h fasted animals and last after administration of 1 h the animals were killed, serum, determination of the activity of serum total cholesterol （T-Cho） concentration and alanine aminotransferase （ALT）, and aspartate aminotransferase （AST）; liver, determination of malondialdehyde （MDA） content in liver, and pathological section. Resuhs： Compared with model group, the high dose and low dose group of blue fox bile powder can significantly decrease thecontent of serum ALT, AST, T-CHO, liver MDA （P〈0.05）, which indicates that the blue fox bile has the function of protecting liver damage caused by D-Galn. Conclusion： The blue fox bile has a protective effect on liver injury induced by D-Gain in mice.