结缔组织生长因子在卵巢癌中的表达及其临床意义

Expression and clinical significance of connective tissue growth factor in ovarian carcinoma

目的：探讨结缔组织生长因子（connective tissue growth factor,CTGF）在卵巢癌组织中的表达及临床意义。方法：采用免疫组化和逆转录聚合酶链反应（RT-PCR）方法检测105例卵巢癌、20例正常卵巢组织和30例良性卵巢肿瘤组织中CTGF的表达,并分析CTGF与卵巢癌临床病理参数的关系。结果：免疫组化检测观察到CTGF主要定位于细胞质内,卵巢癌、正常卵巢组织和良性卵巢肿瘤中CTGF的阳性表达率分别为53.33%（56/105）、95.00%（19/20）、93.33%（28/30）,卵巢癌中CTGF阳性表达率低于正常卵巢（χ^2=12.15,P=0.00）及良性卵巢肿瘤组织（χ^2=15.88,P=0.00）,正常卵巢与良性卵巢肿瘤组织相比较差异无统计学意义（χ^2=0.05,P=0.80）,RT-PCR检测结果显示卵巢癌中CTGF mRNA的表达明显低于正常卵巢组织和良性卵巢肿瘤组织（F=3.39,P=0.039）。进一步采用χ^2检验统计分析得出CTGF与卵巢癌的临床分期、淋巴转移相关（P〈0.05）,而与年龄、病理分级、病理类型及腹水癌细胞阳性率无相关性（P〉0.05）。结论：CTGF在卵巢癌中低表达,与卵巢癌的发生、发展相关。

Objective：To investigate the expression and clinical significance of connective tissue growth factor （CTGF） in ovarian cancer. Methods： The expression levels of CTGF in 20 cases of normal ovarian tissues,30 cases of benign ovanan tumor tissues and 105 cases of ovarian cancer tissues were examined by RT-PCR and immunohistochemical staining, and the correlation between CTGF expression and clinicopathological significance of ovarian cancer was analyzed. Results： The posi- tive rate of CTGF expression in normal ovarian tissues, benign ovarian tumor tissues and ovarian cancer tissues were 95.00% （19/20） ,93.33% （28/30） and 53.33% （56/105）respectively; the positive rate of CTGF in ovarian cancer tissues was sig- nificantly lower than that of normal ovarian tissues（χ2 = 12. 15, P =0.00） and benign ovarian tumor tissues（χ2 = 15.88, P = 0.00） ; there was no significant difference in the positive rate between normal ovarian tissues and benign ovarian tumor tissues（χ2 = 15.88, P =0.00）. The CTGF mRNA expression in ovarian cancer tissue was lower than that in normal ovarian tissue and benign ovarian tumor tissue showed by RT-PCR （ F = 3.39 ,P =0. 039）. Data was processed by χ2 analysis, and the results showed that the expression of CTGF was correlated with clinical stages, lymph node metastasis （ P 〈 0. 05 ）, but not correlated with age, pathologic type,pathologic grade and positive rate of ascites tumor cells （P 〉 0.05 ）. Conclusion： CTGF was low expressed in overian cancer tissues and correlate with the occurrence and progression of ovarian cancer.