FGF-21改善谷氨酸钠引起的体内脂肪堆积

FGF-21 correct the fat accumulation caused by monosodium glutamate

为研究成纤维细胞生长因子-21(FGF-21)对谷氨酸钠(MSG)诱导的肥胖大鼠的脂肪代谢是否具有调节作用,给新生SD大鼠饲喂MSG至20周龄后,连续注射FGF-21(1 mg/kg)32 d,检测FGF-21注射前后大鼠体质量、Lee’s指数、进食量、腹部和皮下脂肪质量、血脂浓度、糖耐量和肝脂代谢中的相关指标.结果显示,与MSG对照组相比,FGF-21试验组大鼠体质量、Lee’s指数、腹部脂肪质量及脂肪细胞大小显著降低(P<0.01),进食量没有明显变化;血糖、TG、TC、LDL-C浓度显著降低(P<0.01),HDL-C浓度升高;脂肪中Glut-1水平显著上升(P<0.05),Leptin mRNA的高表达水平显著下降(P<0.01);肝脏的质量和肝脏中ALT、AST、ALP的水平得到改善,FGF-21、PPARα、PPARγmRNA水平显著下降(P<0.05),脂肪肝得到恢复,糖耐受得到改善.以上结果表明,FGF-21明显改善由MSG诱导引起的体内脂肪堆积,对腹部脂肪的减少效果尤其显著,同时有效改善MSG肥胖大鼠的肝脂代谢水平,恢复脂肪肝.

The paper is to study the effect of fibroblast growth factor-21 （FGF-21） on lipid metabolism in the monosodium glutamate （MSG） induced obese rats. SD rats were induced by MSG after weaning, and then randomly divided into experimental and vehicle groups. After fed to 20 weeks, the experimental groups were injected with FGF-21 （1 mg/kg） for a continuous 32 d. The rats were detected the body weight, Lee＇s index, food intake, abdominal and subcutaneous fat content, lipid content, glucose tolerance and the relevant indicators of hepatic and lipid metabolism before and after the injection. As compared to vehicle-treated rats, FGF-21 did not affect the amount of daily food intake in MSG rats, but led to a significant reduction in Lee＇ s index, total body weight and abdominal fat mass （ 52.8% reduction） at the end of treatment. After FGF- 21 treatment, the levels of glucose, triglycerides, cholesterol and LDL-C decreased except HDL-C. The exogenous FGF-21 led to the improvement of the liver weight, hepatic AST, ALT and ALP and the reduction of endogenous FGF-21, PPARα and PPARγin liver. In summary, FGF-21 could regulate on lipid metabolism and reverse hepatic steatosis in MSG rats.