兔眼小梁切除术后滤过泡组织Fas mRNA的表达

Expression of Fas mRNA of filtration bleb after trabeculectomy in rabbit

目的对小梁切除术后滤过泡组织Fas mRNA的表达进行初步研究。方法新西兰白兔48只单独笼养,随机选取40只兔右眼行小梁切除术,使上方结膜形成滤过泡,按术后时间又分为1 d、7 d、14 d、20 d、28 d共5个亚组,每组8只兔。另外8只兔的右眼未进行手术为正常对照组。在行小梁切除术后1 d、7 d、14 d、20 d、28 d,切下滤过手术区组织,同时于28 d时切除正常对照组相应部位的结膜组织。实时荧光定量PCR检测各组标本的Fas mRNA表达情况。结果兔眼小梁切除术后1 d、7 d、14 d三个亚组Fas mRNA的相对表达量分别为1.33±0.35、1.41±0.28、1.38±0.25,与正常对照组的表达量1.42±0.34相比差异无统计学意义(P>0.05),术后20 d Fas mRNA相对表达量为0.90±0.18,较对照组明显下降,差异有统计学意义(P<0.05),28 d时进一步下降至0.78±0.09(P<0.05)。结论在兔眼结膜滤过泡组织愈合过程中,术后20 d及28 d凋亡主要基因Fas mRNA表达量明显减少,可能为减少青光眼小梁切除术后瘢痕化寻找到一个新的突破口。

Objective To study the expression of Fas mRNA in filtration bleb af- ter trabeculectomy in rabbits. Methods Forty-eight New Zealand rabbits were fed singly, in which 40 cases were randomly chosen to perform the trabeculectomy in the right eye, then the rabbits were sub-divided into 1-day group,7-day group, 14-day group, 20-day group and 28-day group based on the postoperative time,8 cases in each group. Another 8 rabbits received no any operation as the normal control group. At 1 day, 7 days, 14 days,20 days ,28 days after trabeculectomy, the filtration bleb tissues of opera- ted groups were harvested respectively. The conjunctiva tissues of control group were also harvested at 28 days. The expression level of Fas mRNA was detected by Real Time RT-PCR. Results The relative expression of Fas mRNA at 1 day,7 days,14 days after operation were 1.33 ± 0.35, 1. 41 ± 0.28, 1. 38 ±0.25, respectively, compared with the normal control group （ 1.42± 0.34） the difference was not significant （P 〉 0.05 ）. The relative expression of Fas mRNA at 20 days after operation was 0.90 ±0.18 ,which was lower than that in the normal control group （P〈0.05） ,and decreased to 0.78 ±0.09 at 28 days after operation. Conclusion In the procession of filtering bleb tissue healing, the expression of Fas mRNA decrease at 20 days and 28 days, 14 days after operation, which may be emerged as a promising target for treating postoperative scar of trabecu- lectomy.