摘要
随着棉花基因组学、转录组学等重要学科的快速发展,针对候选基因进行序列多样性分析,开发基于候选基因的分子标记,将能够极大推进棉花分子育种研究。本研究利用高通量的测序技术对耐盐陆地棉品种Miscott 7913-83和盐敏感品种苏棉12盐胁迫前后根、叶总RNA的混样进行了转录组测序,对不同盐处理时期Miscott 7913-83和苏12根和叶分别进行表达谱分析。获得3232条盐胁迫下差异表达基因;基于差异表达基因利用生物信息学手段大规模开发了SSR以及In Del等分子标记;随机选择了部分SSR及In Del引物进行验证,进一步证实了分子标记的准确性。本研究为快速开发陆地棉品种间多态性分子标记提供了高效可行的分析方法,通过开发陆地棉耐盐相关功能标记,以期用于分子标记辅助育种改良陆地棉耐盐性。
With the development of cotton genomics and transcriptomics, cotton genetics and breeding would be enhanced by the identification of differentially expressed genes and the development of molecular makers based on candidate genes. The salt-tol- erant cotton variety Miscott 7913-83 and salt-sensitive variety Su 12 were used as experimental materials in this study. RNA samples prepared from the roots and leaves of the two cultivars were pooled for transcriptome sequencing. Variations in gene ex- pression were then examined after exposing the plants to 200 mrnol-L^-1NaCl for 12 and 72 h. A total of 3232 differentially ex- pressed genes were then examined between Miscott 7913-83 and Su 12, and functional molecular markers such as EST-SSR and EST-InDel were designed by informatics tools according to differentially expressed gene sequences. Some SSR and InDel primers were randomly selected and further confirmed the accuracy. This research provides efficient methods for the rapid devel- opment of polymorphic markers in cotton. By focusing on functional molecular markers associated with salt tolerance, this should aid the improvement of salt tolerance by marker-assisted selection in upland cotton.
出处
《棉花学报》
CSCD
北大核心
2016年第1期65-74,共10页
Cotton Science
基金
国家科技重大专项"转基因生物新品种培育"(2014ZX08005-004-002)
棉花生物学国家重点实验室开放课题(CB2015A12)
江苏省自主创新基金(CX(14)2065)
