Calbindin和Parvalbumin在C57BL/6J kit基因突变小鼠听觉传导通路中的表达

The expression of Calbindin and Parvalbumin in auditory pathway of kit gene mutated C57BL /6J mouse

目的：观察钙结合蛋白（CB）和小清蛋白（PV）在C57BL/6J野生型小鼠与kit基因突变型小鼠（kit＋/kitW-2Bao）听觉传导通路上的表达。方法：取kit突变型小鼠和野生型小鼠各6只,水合氯醛腹腔注射麻醉,经心脏灌流固定,取大脑进行冠状位冰冻切片。经免疫组织化学染色,观察CB和PV两种蛋白在小鼠听觉传导通路上的表达差异。结果：PV在野生型小鼠的腹侧耳蜗前核（AVCN）、腹侧耳蜗后核（PVCN）、下丘（IC）以及听皮层（AC）均有明显表达。CB在野生型小鼠的PVCN和斜方体核（Tz）有明显表达,在AC仅在2-3层有极少量表达。kit基因突变引起PV在PVCN、IC和AC的表达减少（P〈0.01）,而Tz的表达增加（P〈0.01）。CB在基因突变小鼠PVCN表达消失,AC的表达增加（P〈0.01）。结论：PV和CB在野生型和突变型小鼠的听觉传导通路的表达存在明显差异。kit基因突变可引起PVCN、IC、Tz和皮层的PV表达变化,PVCN、AC的CB表达变化,表明kit基因突变对听觉通路高级中枢功能活动有一定的影响。

Objective： To observe the expressions of Calbindin（CB） and Parvalbumin（PV）, the two calcium-binding protein, in auditory pathway in mice of wild type C57BL/6J and kit ^＋/kitw^- 2Bao, a kit gene mutant. Methods： Six mutated kit gene kit^＋/kitw^- 2Bao mice and 6 wild type C57BL/6J （B6）mice were anaesthetized i.p. with chloral hydrate. After the mice were fixed by heart perfusion, the brains were removed and coronal sections were cut with a freezing microtome. Results： We found that wild type mice had significant expressions of PV on ventral cochlear nucleus, anterior part （AVCN）, ventral cochlear nucleus, posterior part （PVCN）, inferior colliculus （IC） and auditory cortex （AC）. CB was expressed in wild type mice on PVCN and nucleus of the trapezoid body （Tz）. The mutant of kit gene induced the less expression of PV on PVCN, IC and AC （ P 〈 0.01 ）, but increased the expression of Tz （ P 〈 0.01 ）. CB could not be observed on PVCN in mutant mice, and the expression of AC was increased（ P 〈 0. 01 ）. Conclusion： CB and PV has differential expression level in auditory pathway. Since mutated kit gene can affect expression of PV on PVCN, IC, Tz and AC, as well as CB on PVCN and AC, it suggests that the mutation of kit gene can affect the advanced ftmction of central nervous system in auditory pathway.