母源性甲亢对仔鼠肾甲状腺素受体表达的影响

Effect of maternal hyperthyroidism on expression of thyroid hormone receptor in kidney of postnatal rat

目的：研究母源性甲亢对新生仔鼠肾的影响。方法：建立妊娠合并甲亢模型，取新生1、5、10和15d仔鼠肾。分别进行解剖学及组织病理检查，采用实时定量PCR法检测甲状腺激素受体（TR）TRα1、TRα2、TRβ1在肾组织中mRNA水平表达量的变化。结果：母源性甲亢仔鼠与同期对照仔鼠相比，光镜结构未见明显异常；甲亢组TRα1的表达量略高于对照组（仔鼠1d上调约50％，仔鼠5d上调约32％，仔鼠15d上调约48％）；甲亢组TRα2的表达量略高于对照组（仔鼠1d上调约36％，仔鼠10d上调约33％）；TRβ1的表达差异无统计学意义。结论：母源性甲亢引起甲状腺激素受体的差异表达，以TRα1、TRα2的变化为主，可能在甲亢引起的子代肾损伤中扮演重要角色。

Objective： To study effect of maternal hyperthyroidism on kidneys of postnatal rats. Methods： Hyperthyroidism model of rats was set up and kindey of postnatal rats on 1,5, 10, 15 days were harvested, rnRNA expression of thyroid hormone receptors（TR）α1, TRα2 and TRβ1 was examined by RQ-PCR. Results： Light microscope of neonatal kidneys showed no significant difference between maternal hyperthyroidism and normal ones. The expression of TRα1 increased by 50G on the 1st day, 32G on the 5th day and 48% on the 15th day compared to normal group. The expression of TRα2 increased by 36% on the 1st day and 33% on the 10th day compared to normal group. The expression of TRβ1 showed no difference between hyperthyroidism and normal group. Conclusion： The maternal hyperthyroidism can influence the differential gene expression of thyroid hormone receptors, mainly in TRα1, and TRα2, which may play important role in offspring renal injury.