黄芪甲苷对成年大鼠视神经切断后视网膜节细胞存活的影响

Effects of astragaloside on survival of retinal ganglion cells in adult rats with optic nerve transection

目的：探讨黄芪甲苷（AST）在成年大鼠视神经切断后对视网膜节细胞（RGCs）存活的影响。方法：动物分为正常组、单纯切断视神经组、AST处理组和生理盐水对照组。用荧光金（FG）逆行示踪标记法及定量解剖学技术观察正常和经AST处理的SD大鼠于视神经切断后5、7、14d的RGCs的密度。结果：正常组RGCs平均密度为（2230±156）／mm^2。单纯视神经切断组RGCs平均密度与生理盐水对照组相比较，无显著性差异；AST处理组与单纯切断视神经组和生理盐水对照组相比较，在各个时间点上均存在显著性差异。结论：黄芪甲苷可提高成年大鼠视神经切断后视网膜节细胞短期存活。

Objective： To investigate the effect of astragaloside IV （AST） on the survival of retinal ganglion cells after optic nerve cut in adult rats. Methods： Animals were divided into normal group, simple optic nerve transection group, AST treatment group and saline control group. Fluorogold （FG） retrograde tracing and quantitative anatomical technique were used to observe RGCs density of rats in normal and AST treatment group at 5,7 days and 14 days. Results： The average density of RGCs was （2 230±156）/mm^2 in normal group. There were no significant differences in RGCs average densities between simple optic nerve transection group and saline control group; Comparison among AST treatment group with the normal group and saline control group showed there were significant differences at each time point. Conclusion： Astragaloside IV can improve short-term survival of RGC after transection of the optic nerve in adult rats.