基于核磁共振定量氢谱技术的乳品脂质分析及方法建立

New approach for determination of lipids in different milk matrix by using quantitative ^1H NMR

基于核磁共振定量氢谱(q1H NMR)技术,建立了乳品脂质分析的方法,并对市场上有机牛奶、山羊奶、大豆奶中的ω-3,DHA,EPA等脂质进行定量分析。就ω-3而言,有机牛奶>山羊奶>大豆奶;就DHA而言,山羊奶>有机牛奶>大豆奶;大豆奶中不含EPA,有机牛奶中EPA>山羊奶。此外,对不同种类奶中脂质的积分数据进行偏最小二乘判别分析(PLS-DA)及方差分析(ANOVA),进一步明确了其脂质种类及含量分布的差异,并对其进行初步营养学评价。

A new approach for determination of lipids in different milk matrix by using quantitative 1 H NMR was established. The organic bovine milk, goat i milk and soymilk collected from supermarket were detected. The results showed that the order of ω-3 was organic bovine milk 〉 goat milk 〉 soymilk, and the order of DHA% was goat milk 〉 organic bovine milk 〉 soymilk. The percentage of EPA in organic bovine milk was higher than that in goat milk, and no EPA was detected in soymilk. Besides, the PLS-DA and ANOVA methods were used to further analyze the difference of lipid distribution in the three species of milk and then evaluate the nutrition value