右美托咪定对异丙酚孵育胎鼠离体海马神经元NGF表达的影响

Effect of dexmedetomidine on expression of NGF in isolated hippocampal neurons of fetal rats incubated with propofol

目的 评价右美托咪定对异丙酚孵育离体胎鼠海马神经元神经生长因子（NGF）表达的影响.方法 原代培养孕18 dSD大鼠胎鼠海马神经元7d,以5×10^5个/ml的细胞密度接种于培养板,采用随机数字表法分为3组（n=15）：对照组（C组）不做任何处理;异丙酚组（P组）加入异丙酚,终浓度100 μmol/L;右美托咪定＋异丙酚组（DP组）加入右美托咪定,终浓度1μmol/L,孵育30min,随后加入异丙酚,终浓度100 μmol/L,孵育3h.采用CCK-8法检测海马神经元活力,RT-PCR法检测NGF mRNA的表达,Western blot法检测NGF蛋白的表达.结果 与C组比较,P组海马神经元活力降低,NGF蛋白及其mRNA表达下调（P＜0.05）;与P组比较,DP组海马神经元活力升高,NGF蛋白及其mRNA表达上调（P＜0.05）.结论 右美托咪定通过上调NGF表达改善异丙酚诱导的胎鼠离体海马神经元活力降低.

Objective To evaluate the effect of dexmedetomidine on the expression of nerve growth factor （NGF） in isolated hippocampal neurons of fetal rats incubated with propofol.Methods Hippocampal neurons derived from the fetal rats of pregnant Sprague-Dawley rats at 5-13 days of gestation were primarily cultured for 7 days,and were inoculated in the culture plate at a density of 5×10^5 cells/ml.The neurons were randomly divided into 3 groups （n =15 each） using a random number table：control group （group C）,propofol group （group P）,and dexmedetomidine ＋ propofol group （group DP）.In group P,propofol with the final concentration of 100 μmol/L was added to the culture medium,and the cells were incubated for 3 h.In group DP,dexmedetomidine with the final concentration of 1 μmol/L was added to the culture medium,the cells were incubated for 30 min,and then propofol with the final concentration of 100 μmol/L was added to the culture medium,and the cells were incubated for 3 h.The viability of hippocampal neurons was assessed by CCK-8 assay.NGF mRNA expression was detected by real-time reverse transcriptase polymerase chain reaction.NGF protein expression was detected by Western blot.Results Compared with group C,the viability of hippocampal neurons was significantly decreased,and the expression of NGF protein and mRNA was down-regulated in group P （P〈0.05）.Compared with group P,the viability of hippocampal neurons was significantly increased,and the expression of NGF protein and mRNA was up-regulated in group DP （P〈0.05）.Conclusion Dexmedetomidine improve propofol-induced decrease in the viability of isolated hippocampal neurons of fetal rats through up-regulating the expression of NGF.