火把花根对急性肺损伤大鼠肾组织紧密连接蛋白claudin-5及ZO-1表达的影响

Effect of colquhounia root tablet on expression of tight junction proteins claudin-5 and ZO-1 in renal tissues of rats with acute lung injury

目的 评价火把花根对急性肺损伤大鼠肾组织紧密连接蛋白claudin-5及ZO-1表达的影响.方法 清洁级健康雄性SD大鼠24只,12～13周龄,体重250～ 300 g,采用随机数字表法分为3组（n=8）：对照组（C组）、急性肺损伤组（ALI组）和火把花根组（H组）.采用尾静脉注射油酸0.04 ml/kg的方法制备大鼠急性肺损伤模型.H组尾静脉注射前给予火把花根片600 mg·kg-1·d-1溶液连续灌胃10d,第10天灌胃后1h尾静脉注射油酸,6h后采集腹主动脉血样,测定血浆肌酐（Cr）和尿素氮（BUN）浓度,随后处死大鼠取肺和肾,计算肺湿重/干重（W/D）比值,观察肺组织病理学结果,采用免疫组化法和Western blot法检测肾组织claudin-5和ZO-1的表达,光镜下观察肾组织病理学结果,并行肾小管损伤评分（RTDS）.结果 与C组比较,ALI组肺W/D比值、血浆Cr和BUN浓度、RTDS升高,肾组织claudin-5和ZO-1表达下调（P＜0.01）;与ALI组比较,H组肺W/D比值、血浆Cr和BUN浓度、RTDS降低,肾组织claudin-5和ZO-1表达上调（P＜0.01）.H组肺组织和肾组织病理学损伤较ALI组明显减轻.结论 火把花根可通过上调肾组织紧密连接蛋白claudin-5及ZO-1的表达减轻急性肺损伤大鼠的肾损伤.

Objective To evaluate the effect of colquhounia root tablet on the expression of tight junction proteins claudin-5 and ZO-1 in the renal tissues of rats with acute lung injury （ALI）.Methods Twenty-four healthy male Sprague-Dawley rats,weighing 250-300 g,were randomly divided into 3 groups （n =8 each） using a random number table：control group （group C）,ALI group;and colquhounia root tablet group （group H）.Oleic acid 0.04 ml/kg was injected into the trail vein to establish the model of ALI.In group H,colquhounia root tablet 600 mg · kg-1 · d-1 was instilled into the stomach of each rat for 10 consecutive days,and oleic acid was injected at 1 h after instillation on 10th day.Blood samples were collected from the abdominal aorta at 6 h after injection of oleic acid for determination of plasma creatine （Cr） and blood urine nitrogen （BUN） concentrations.The animals were then sacrificed.The lungs were removed for determination of wet/dry lung weight ratio （W/D ratio）,and for examination of the pathological changes.The kidneys were removed for determination of the expression of claudin-5 and ZO-1 （by immunohischemistry assay and Western blot） and for examination of the pathological changes.The damage to the renal tubules was scored.Results Compared with group C,W/D ratio,plasma Cr and BUN concentrations,and renal tubular damage score were significantly increased,and the expression of claudin-5 and ZO-1 was down-regulated in group ALI （P〈0.01）.Compared with group ALI,W/D ratio,plasma Cr and BUN concentrations,and renal tubular damage score were significantly decreased,and the expression of claudin-5 and ZO-1 was up-regulated in group H （P〈0.01）.The pathological changes of lungs and kidneys were significantly attenuated in group H as compared with group ALI.Conclusion Colquhounia root tablet can attenuate kidney injury through up-regulating the expression of tight junction proteins claudin-5 and ZO-1 in the renal tissues of rats with ALI.