炮制对王不留行中王不留行环肽A,B,E含量的影响

Effects of Processing on Contents of Segetalin A,B,E in Vaccariae Semen

目的:研究炮制对王不留行中王不留行环肽A,B,E含量的影响,探索王不留行的炮制机制。方法:建立高效液相色谱法(HPLC)同时测定王不留行中王不留行环肽A,B,E含量的方法,采用Acclaim^(TM)120 C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-水(B)梯度洗脱(0~8 min,32%A;8~13 min,32%~60%A;13~20 min,60%~70%A),流速1 m L·min^(- 1),检测波长220 nm,柱温40℃,进样量10μL。分析炮制前后王不留行环肽A,B,E含量及溶出率的变化。结果:炮制前后,王不留行环肽A,B,E在王不留行中含量差异较小,炮制前分别为0.035%,0.009%,0.017%,炮制后分别为0.033%,0.009%,0.015%;但3个成分的水煎溶出率分别升高了13.2%,5.3%,17.4%。结论:炮制对王不留行环肽A,B,E含量的影响较小,但炮制后3个成分的水煎溶出率均有增加。

Objective： To investigate influence of processing on contents of segetalin A, B, E in Vaecariae Semen. Method： HPLC was employed to determine contents of segetalin A, B, E with AcclaimTM 120 C18 column （4. 6 mm ×250 mm, 5 μm）, mobile phase of acetonitrile （A） -water （B） for gradient elution （0-8 min, 32%A; 8-13 min, 32%-60%A; 13-20 min, 60%-70%A）, flow rate of l mL＇min-1, detection wavelength at 220 nm, column temperature at 40℃ , injection volume of 10μL. Changes of contents of segetalin A, B, E and dissolution rates were analyzed after Vaccariae Semen being processed. Result： After being processed, contents of segetalin A, B, E in processed products of Vaccariae Semen was similar to those of raw herbs, however, their dissolution rates raised 13.2% , 5.3% and 17.4% , respectively. Conclusion： Processing has little influence on contents of segetalin A, B, E in Vaccariae Semen, but their dissolution rates increase.