不同产地连翘挥发油主要成分分析及抗菌活性研究

Analysis of Major Components and Antibacterial Activity of Volatile Oil from Forsythiae Fructus in Different Origins

目的:进行不同产地连翘挥发油的主要成分气相测定及其体外抗菌作用的比较研究。方法:采用水蒸气蒸馏法提取不同产地16批连翘果实中的挥发油,利用气相色谱分析检测其主要成分及含量,采用K-B纸片扩散法比较分析其抗菌活性。色谱条件为HP-5毛细管色谱柱(0.25 mm×30 m,0.25μm),程序升温为初始温度50℃,以5℃·min^(- 1)的速率升至86℃,保持3 min,再以20℃·min^(- 1)的速率升至220℃保持1 min,进样口温度230℃,检测器温度250℃。结果:α-蒎烯,莰烯,β-蒎烯,月桂烯,对伞花烃,柠檬烯和α-松油醇的线性关系良好(r均>0.999 7),线性范围分别为0.016 5~0.988 8,0.005~0.300,0.038 1~2.286 0,0.003 2~0.192 0,0.000 8~0.048 0,0.008 2~0.492 0,0.002 4~0.144 0μg;稳定性、重复性试验的RSD均<3.0%;加样回收率为99.0%~103.4%(RSD<3.0%);不同产地连翘挥发油中主要成分含量有差异,其对5种试验菌均有不同程度抑制作用。结论:所建立的连翘挥发油多成分GC测定方法简便、准确,可用于连翘药材的质量评价;抗菌实验结果可靠,可用于连翘药材的抗菌作用评价。

Objective： To determine the major components of volatile oil from Forsythiae Fructus in different origins by gas phase method, and compare its antibacterial activity in vitro. Method： Volatile oil of 16 batches of Forsythiae Fructus in different origins were extracted by steam distillation method ; the major components and content were detected and analyzed by gas chromatography （GC） ; their antibacterial activity was analyzed and compared by K-B disk diffusion method. Chromatographic conditions： HP-5 capillary chromatographic column （0.25 mm×30 m, 0.25 μm）. Procedure temperature： initial temperature of S0 , and increased to 86℃ at the rate of 5 ℃· min-l, kept for 3 min, and then increased to 220 ℃ at the rate of 20℃·min-1, and kept forl rain. Injection port temperature was 230℃ , and detector temperature was 250℃. Result： a-pinene, camphene, β-pinene, myrcene, p-cymene, limonene, and a-terpineol had good linear relationship within the range of 0.016 5-0.988 8, 0.005 0-0.300 0, 0.038 1-2.286 0, 0.003 2-0. 192 0, 0.000 8-0.048 0, 0.008 2-0.492 0 and 0. 002 4-0. 144 0μg （r〉 0. 999 7） respectively; RSDs of the stability test and reproducibility test were less than 3.0%; the recovery rate of the method was in the range of 99.0% -103.4% （RSD 〈 3.0%）; there were differences in the contents of the major components of volatile oil from Forsythiae Fructus in different origins, and the volatile oil had different inhibitory effect against five kinds of the tested strains. Conclusion： The established GC method is simple, accurate and reliable, and can be used for quality evaluation and antibacterial activity evaluation of Forsythiae Friuctus.