肾安颗粒对5/6肾切除模型大鼠骨代谢的影响

Effect of Shen'an Granule on Bone Metabolism in 5/6 Nephrectomy Model Rats

目的:探讨肾安颗粒对5/6肾切除模型大鼠骨代谢的影响。方法:应用5/6肾切除建立大鼠肾性骨病(ROD)模型,随机分为正常组,假手术组,模型组,阳性药骨化三醇组(1.5μg·kg^(-1)),肾安颗粒低、高剂量组(2.25,4.5 g·kg^(-1)),给予相应药物ig 8周,给药前、后检测大鼠血尿素氮(BUN),血肌酐(SCr),血红蛋白(HGB),钙(Ca),磷(P),碱性磷酸酶(ALP),化学发光法测全段甲状旁腺素(i PTH),双能X射线测骨密度。结果:与正常组比较,模型组大鼠血BUN,SCr水平明显升高(P<0.05),HGB,血Ca,骨密度明显降低(P<0.05),血P,ALP,全段i PTH明显升高(P<0.05)。治疗后与模型组比较,肾安颗粒2.25,4.5 g·kg^(-1)组大鼠血BUN,SCr水平明显降低(P<0.05),HGB,血Ca,骨密度明显升高(P<0.05),血P,ALP,全段i PTH明显降低(P<0.05)。结论:肾安颗粒能明显改善5/6肾切除模型大鼠肾功能和骨代谢指标,延缓肾功能进展和减轻并发症。

Objective： To investigate the effect of Shen＇an granule on bone metabolism in 5/6 nephrectomy model rats. Method： Renal osteodystrophy （ROD） rat models were established using 5/6 nephrectomy. The rats were randomly divided into normal group, sham operation group, model group, positive drug calcitriol group （ 1.5 μg ·kg-1 ） , Shen＇an granules low dose group and high dose group （2.25, 4.5 g ·kg-1 ）. Corresponding drugs were given by gavage for 8 weeks. Before and after treatment, blood urea nitrogen （BUN） , serum creatinine （SCr）, hemoglobin （HGB）, calcium （Ca）, phosphor （P）, and alkaline phosphatase （ALP） were detected; intact parathyroid hormone （iPTH） was detected with chemiluminescence method, and bone density was measured with dual energy X ray. Result： Compared with the normal group, BUN and SCr levels of ROD model rats were significantly increased （P 〈 0.05 ）, HGB, serum Ca and bone density levels were significantly decreased （P 〈 0.05） , serum P, ALP and whole iPTH levels were significantly increased （ P 〈 0.05）. Compared with the model group, BUN and SCr levels in Shen＇an granule 2.25, 4.5 g ·kg-1dose groups were significantly decreased （P 〈 0. 05）, HGB, serum Ca and bone density levels were significantly increased （P 〈0.05）, serum P, ALP and whole iPTH levels were significantly decreased （P 〈0. 05）. Conclusion： Shen＇an granule can significantly improve the renal function and bone metabolism indexes in 5/6 nephrectomy model rats, and delay progression of renal function and reduce the complications.