益肾降浊化瘀方及其拆方对肾间质纤维化大鼠TNF-α及IL-8表达的影响

Effects of Yishen Jiangzhuo Huayu Decoction and Disassembled Formulas on TNF-α and IL-8 in Rats With Renal Interstitial Fibrosis

目的:观察益肾降浊化瘀方及其拆方对腺嘌呤致肾间质纤维化模型大鼠肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)表达的影响,探讨益肾降浊化瘀方对肾间质纤维化的作用机制。方法:将SPF级雄性SD大鼠70只分正常组,模型组,尿毒清组,全方组,拆方1组,拆方2组,拆方3组,除正常组比较,用腺嘌呤ig法制作肾间质纤维化模型。正常组,模型组予生理盐水(10 m L·kg^(-1)·d^(-1)),尿毒清组(2.5 g·kg^(-1)·d^(-1)),全方组(20.2 g·kg^(-1)·d^(-1)),拆方1,2,3组(11,8.2,7.9 g·kg^(-1)·d^(-1))。给药4周后,酶联免疫吸附法(Elisa法)检测血清TNF-α,IL-8含量,免疫组织化学法检测肾组织中TNF-α,IL-8蛋白水平。结果:与正常组比较,模型组TNF-α和IL-8在血清和肾组织中含量明显升高(P<0.05);与模型组比较,全方组、拆方1组、拆方2组、拆方3组血清和肾组织TNF-α,IL-8含量明显降低(P<0.05),其中全方组肾组织TNF-α,IL-8蛋白表达和血清TNF-α,IL-8含量明显低于各拆方组(P<0.05)。结论:益肾降浊化瘀方全方组及其拆方1组、拆方2组、拆方3组均能下调TNF-α,IL-8的表达,其中以益肾降浊化瘀方全方组作用最显著。

Objective： To observe the effect of Yishen Jiangzhuo Huayu decoction and disassembled formulas on expression of tumor necrosis factor-α （TNF-α） and interleukin-8 （IL-8） in rats with adenine-induced renal interstitial fibrosis, and explore the mechanism of Yishen Jiangzhuo Huayu decoction on renal interstitial fibrosis. Method： Totally 70 SPF male SD rats were divided into normal group, model group, Niaoduqing group, whole prescription group, disassembled formulas group 1, group 2, and group 3. All the rats except normal group were given intragastrically with adenine to produce renal interstitial fibrosis models. The normal group and model group received normal saline solution （ 10 mL· kg-1· d-1 ） , Niaoduqing group （ 2.5 g · kg-1· d-1 ） , whole prescription group （20.2 g· kg-1·d-1）, the disassembled formulas groups 1, 2, 3 （11, 8.2, 7.9 g·kg-1· d-1 ）. 4 weeks after administration, enzyme-linked immunosorbent assay （Elisa method） was used to detect TNF-α, IL-8 content in serum, and immunohistochemistry assay was used to detect TNF-α, IL-8 protein levels in renal tissues. Result： Compared with the normal group, TNF-α and IL-8 levels in serum and renal tissues were significantly higher in model group （P 〈 0.05）. Compared with the model group, TNF-α and IL-8 levels in serum and renal tissues were significantly lower in the whole prescription group, disassembled formulas group 1, group 2 and group 3 （P 〈 0.05） ; TNF-α, IL-8 protein expression in renal tissues as well as TNF-α and IL-8 levels in serum of the whole prescription group were significantly lower than each disassembled formula group （ P 〈 0.05）. Conclusion ： Yishen Jiangzhuo Huayu decoction whole prescription group, disassembled formula group 1, group 2, and group 3 could down-regulate TNF-α, IL-8 expression, where the whole prescription group had the most significant effect.