螃蟹甲大孔吸附树脂50%乙醇洗脱物对大鼠CCl_4肝损伤的保护作用

Hepatoprotective Effects of 50% Ethanol Elute of Phlomis younghunsbandii by Macroporous Adsorptive Resins on CCl_4-induced Acute Liver Injury

目的:探讨螃蟹甲中大孔吸附树脂50%乙醇洗脱物对四氢化碳(CCl_4)致急性肝损伤的保护作用及其可能机制。方法:60只Wistar大鼠随机分为正常组,模型组,联苯双酯组(联苯双酯滴丸100 mg·kg^(-1)),螃蟹甲大孔吸附树脂50%乙醇洗脱物(EPY-50)高、中、低剂量组(400,200,50 mg·kg^(-1)),连续ig给药4 d,正常组和模型组均ig给予纯化水,第3天给药1 h后,ip 15%CCl_4橄榄油,正常组ip橄榄油,染毒2,22 h后再次给药,末次给药1 h后,眼眶静脉丛采血,测血清中丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),丙二醛(MDA),超氧化物歧化酶(SOD)的水平,处死大鼠剖取肝脏,测肝指数及肝组织中MDA,SOD,谷胱甘肽还原酶(GSH)含量,并做肝脏组织病理学检查。结果:与正常组比较,模型组大鼠血清中AST,ALT水平显著增加(P<0.01),肝组织匀浆液中的MDA含量明显升高(P<0.05),SOD,GSH含量均明显降低(P<0.05),肝脏指数显著增加(P<0.01),表明CCl_4造模成功。与模型组比较,EPY-50高、中剂量组能显著降低CCl_4致急性肝损伤大鼠血清中AST,ALT水平(P<0.05,P<0.01),并显著减少肝组织中MDA含量,提高SOD,GSH的含量,降低肝指数(P<0.05,P<0.01),改善肝组织病理状态。结论:EPY-50对大鼠CCl_4致急性肝损伤有保护作用,其作用机制可能与其抗氧化活性有关。

Objective： To explore the hepatoprotective effects and the possible mechanism of 50% ethanol elute of Phlomis younghunsbandii by macroporous adsorptive resins （EPY-50） on CC14-induced acute liver injury rats. Method： Sixty Wistar rats were randomly divided into normal group, model group, bifendate group （bifendate dropping pill, 100 mg·kg-1） and EPY-50 high-dose, medium-dose and low-dose （400, 200, 50 mg·kg-1） groups, continuous for 4 days by gavage. The rats in normal group and model group were given with distilled water. 1 hour after administration on day 3, rats were ip injected with 15% CC14 olive oil; rats in normal group were ip injected with with olive oil, After contamination for 2 h and 22 h, all groups were administered again and blood was taken from orbital vein at 1 h after the last administration. The levels of serum aspartate aminotransferase （AST） , alanine aminotransferase （ALT） , malondialdehyde （MDA） , and superoxide dismutase （SOD） were detected, then the rats were sacrificed for and livers were taken. The liver index and MDA, SOD, and glutathione reductase （GSH） levels in the liver tissues; in addition, histopathological examination was also done for the livers. Result： Compared with the normal group, serum AST and ALT levels were significantly elevated in model group （P 〈 0.01 ） , MDA levels were significantly increased （P 〈 0.05） , SOD and GSH levels were statistically reduced in the liver tissues （ P 〈 0.05 ）, the liver index was significantly increased （ P 〈 0.01 ）, indicating that the model of CC14 injection was successful. Compared with the model group, EPY-50 with high- dose, and medium-dose （400, 200 mg·kg-1） could significantly decrease the levels of AST, ALT （P 〈 0.01, P 〈 0.01 ） , significantly decrease MDA level, increase the SOD and GSH levels, reduce liver index and improve pathological state of the liver tissues. Conclusion： EPY-50 has hepatoprotective effects on CC14-induced acute liver injury, and the mechanism may be associated with its antioxidative activity.