转基因抗虫耐除草剂复合性状玉米‘双抗12-5’对亚洲玉米螟的抗性及对草甘膦的耐受性研究

Evaluation of transgenic maize‘Shuangkang 12-5' with complex traits of insect-resistance and glyphosate-resistance for the resistance to Ostrinia furnacalis and tolerance to glyphosate

通过田间接虫、室内生测以及田间植株喷施草甘膦试验,在吉林省公主岭市综合评价了转cry1Ab/cry2Aj和G10evo-epsps基因抗虫耐除草剂复合性状玉米‘双抗12-5’对亚洲玉米螟[Ostrinia furnacalis（Guenée）]的抗性以及对草甘膦的耐受性,为‘双抗12-5’在吉林省产业化应用提供基础科学数据。结果表明,与非转基因对照‘郑单958’相比,‘双抗12-5’全生育期对亚洲玉米螟都具有良好的抗性,达到高抗水平;取食‘双抗12-5’心叶、雄穗及花丝等组织7d后的亚洲玉米螟幼虫存活率为4%~12%,而取食‘郑单958’各个组织的亚洲玉米螟幼虫存活率均高于96%。‘双抗12-5’在喷施推荐剂量（1 230g/hm2）及在2倍推荐剂量（2 460g/hm2）草甘膦情况下,4周内均生长正常,受害率为0;对照‘郑单958’植株第一周即表现出明显的药害症状,逐渐枯萎死亡,受害率达到100%。综上所述,‘双抗12-5’在吉林省表现出良好的抗虫性和除草剂耐受性。

‘Shuangkang 12 - 5＇ is a maize variety harbored the genes crylAb/cry2Aj and GlOevo-epsps. Evalua- tion of the transgenic maize for the resistance to Asian corn borer [Ostrinia furnacalis （Guence）] and tolerance to glyphosate was assayed in Gongzhuling City, Jilin Province. Field and indoor bioassay demonstrated that the‘Shuangkang 12-5＇ exhibited a high resistance to Asian corn borer during the whole growth period, compared with non-transgenic variety ‘Zhengdan 958＇. The survival rate of the larvae that fed on different tissues of ‘Shuang- kang 12-5＇ for 7 days were 4%--12%, while that fed on non-transgenic lines were above 96 %. When treated with glyphosate at the recommended dose （1 230 g/hm2） and the double dose （2 460 g/hmZ）, all the transgenic lines survived in four weeks, but there was symptom of serious chemical damage in one week and the plant gradually died for the non-transgenic lines. Thus, the transgenic maize ’Shuangkang 12-5 ＇ presented good insect resistance and herbicide tolerance in Jilin Province.