变性梯度凝胶电泳分析亚麻茎秆菌群多样性

Analysis of Flax Stem Microflora Community Diversity by Denaturant Gradient Gel Electrophoresis

亚麻是中国重要的经济作物,为了提高亚麻原茎脱胶的效率,在利用PCR-DGGE技术研究亚麻茎杆上菌群多样性的基础上,对其最优电泳条件进行了优化。结果表明：当使用经胶回收后的样品进行PCR-DGGE,电泳时间为200~220 min,变性凝胶梯度为25%~55%,固定时间为12~15 min,染色时间为20 min,显影时间为5~10 min时,得到电泳和分离效果最好。笔者通过优化DGGE电泳条件,可以更加清晰的得到沤麻过程中细菌的多样性,该工作对利用PCR-DGGE技术研究亚麻茎秆菌群多样性提供了方法基础。

Flax is an important economic crop in China. In order to improve the efficiency of retting, theauthors used PCR- DGGE technique to study the flax stem microflora diversity, and optimized the bestcondition of this method. The results showed that, when PCR-DGGE was carried out using the gel recyclingsamples, the electrophoresis time was 200- 220 min, modified gel gradient was 25%- 55%, fixed time wasabout 12-15 min, dyeing time was 20 min, developing time was 5-10 min, the electrophoresis and separationresults were good. The study clearly obtained the bacteria diversity in flax retting by optimizing theelectrophoresis condition of DGGE. It could set a base for the use of PCR- DGGE to study the microfloracommunity diversity of flax stem.