高效液相色谱法测定银花泌炎灵片中野黄芩苷含量

Content Determination of Scutellarin in Yinhua Miyanling Tablets by HPLC

目的建立测定银花泌炎灵片中野黄芩苷含量的高效液相色谱法。方法色谱柱为Kromasil C18柱（250 mm×4.6 mm,5μm）,流动相为甲醇-0.4%磷酸溶液（15∶85）,检测波长为335 nm,柱温35℃,流速0.8 m L/min。结果野黄芩苷进样量在0.027～0.216μg范围内与峰面积呈良好的线性关系（r=1.000 0）,平均回收率为97.35%,RSD为0.56%（n=6）。结论该法简便、准确、专属性强、分离效果好,可用于银花泌炎灵片的质量控制。

Objective To establish an HPLC method to determine the content of scutellarin in Yinhua Miyanling Tablets. Methods The HPLC method was adopted. The determination was performed on the Kromasil- Cls column （250 mm×4.6 mm, 5 μm） with the mobile phase of methanol-0.4% phosphoric acid（15： 85） at the flow rate of 0. 8 mL/min. The column temperature was 35~C. The detection wavelength was 335 nm. Results The injection volume of scutellarin showed good linearity with the peak area in the range of 0. 027- 0. 216 μg（ r = 1. 000 0）,the average recovery rate was 97.35%, RSD was 0.56% （n =6）. Conclusion The established method is Simple, accurate and strongly specific with satisfactory separation results, which can be used for the quality control of Yinhua Miyanling Tablets.