摘要
为探讨赤芍水煎剂在体外对金黄色葡萄球菌增殖及细菌毒力因子(α-溶血素、肠毒素)分泌的影响,在体外以不同浓度的赤芍水煎剂作用于金黄色葡萄球菌,记录细菌的生长曲线;用Western-blot方法检测经赤芍水煎液处理后的细菌培养液上清中细菌主要的毒力因子α-溶血素、肠毒素A、肠毒素B的含量。结果表明赤芍水煎剂在0~10 mg/m L对金黄色葡萄球菌ATCC29213和ATCC43300菌株的增殖没有明显的影响,但是赤芍水煎剂在不抑制细菌增殖的浓度下,能够降低金葡菌ATCC29213和ATCC43300菌株毒力因子α-溶血素、肠毒素A、肠毒素B的分泌表达。本研究结果提示赤芍水煎液中具有抗细菌毒力因子的药物成分。
To study the influence of Radix paeoniae Rubra decoction on the secretion of virulence factors(α-Hemolysin, HLα; staphylococcal enterotoxins) and the growth of Staphylococcus aureus in vitro, Staphylococcus aureus was treated with Radix paeoniae Rubra decoction at different concentrations. Then the bacterial growthcurve was recorded. Western-blot was performed to investigate the content of HLα, staphylococcal enterotoxinsA(SEA) and B(SEB) in the supernatant of bacterial culture solution. The results showed that Radix paeoniae Rubra decoction could not inhibit the proliferation of Staphylococcus aureus(ATCC29213 and ATCC43300)obviously when the concentration ranged from 0 to 10 mg/m L. However, it could decrease the secretion of HLα,SEA and SEB in a dose-dependent manner. The results indicated that there was a potential anti-virulenceingredient in Radix paeoniae Rubra decoction.
出处
《中国农学通报》
2016年第5期28-32,共5页
Chinese Agricultural Science Bulletin
基金
科研基地建设科技创新平台"中药方剂中抗细菌溶血素成分及机制研究"(PXM2014_014207_000026)
北京农学院科技创新团队科研能力提升工程"几种中兽药抗病机理的研究"(KCT2014012)
国家自然基金青年基金"小檗碱抗炎新机制―调节MD2的表达和功能"(31302137)
关键词
赤芍
金黄色葡萄球菌
α-溶血素
肠毒素
Radix paeoniae Rubra
Staphylococcus aureus
α-Hemolysin
staphylococcal enterotoxins