大鼠肺爆震伤模型的建立与伤情分析

Establishment of the blast pulmonary injury model and injury evaluation of rats

目的建立密闭胸部爆震伤模型,并探讨其作用于实验动物的相关病理生理变化。方法用爆震伤模型模拟爆炸致伤,根据所受冲击波压强的不同,将所有大鼠按数字表法随机分为3组。A组:正常对照组,未给予爆炸处理;B组:采用额定1.5个标准大气压上限密闭爆炸处理;C组:采用额定3个标准大气压上限密闭爆炸处理。每组20只。爆炸后统计大鼠的生存率及死亡率,检测血炎性因子的变化情况,观察肺病理改变等。结果 C组大鼠死亡率(40%)、病理损伤程度及炎性因子的含量明显高于B组(25%)和A组(0)。结论在一定大气压的密闭环境下.大鼠肺部爆震伤伤情随压强的增大而加重。死亡率随体内肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)的量的增加而增加,二者亦随冲击波压强的增大而呈相关性增长。

Objective To establish a closed blast thoracic injury model in rats and investigate pathophysiologieal changes ex- erted on the experimental animals. Methods The blast injury model was developed with simulated explosions in various explosive scales. In accordance with different scales, the rats were divided into 3 groups, each consisting of 20 animals. Group A （ or the control group） was left intact without any blast treatment; Group B was treated with the rated 1.5 ATA internal blast and Group C was treated with the rated 3.0 ATA internal blast. Following blast treatment, the mortality rates and survival rates of the experimental animals were statistically analyzed, and changes in the serum inflammatory factor were detected and pathological changes of the lungs were observed. Results No injury occurred to the rats in group A, The mortality rate, the seriousness of pathological injury and the level of inflamma- tory factor in the animals of group C were significantly higher than those of the animals in group A and group B. Conclusion Under a certain overpressure in a closed environment, the seriousness of lung blast injury in rats aggravated with the increase of pressure. Mor- tality rates of the exposed animals increased with the elevated levels of tumor necrosis factor-α （TNF-α） and Interleukin-6 （IL-6）. The levels of TNF-α and IL-6 also were also associated with the increase of the blast pressure in the body,