口腔鳞癌患者与健康人外周血清差异表达蛋白的初步研究

A primary study of differentially expressed proteins in the serums of healthy people and patients with oral squamous cell carcinoma

目的通过口腔鳞癌患者血清及健康人血清中蛋白质组成的对比分析,获得口腔鳞癌患者血清中的蛋白变化。方法口腔鳞癌患者及健康人外周血清各20例,通过固相p H梯度双向凝胶电泳以及质谱鉴定和生物信息学分析,寻找在口腔鳞癌中差异表达的蛋白质。结果口腔鳞癌和正常人血清差异表达明显的7个点,对其进行质谱鉴定和生物信息学分析,鉴定了其中的一个点,为Chain B,T-To-T(High)Quaternary Transitions In Human Hemoglobin.在口腔鳞癌血清中均为低表达。结论Chain B,T-To-T(High)Quaternary Transitions In Human Hemoglobin:Deshis146beta Deoxy Low-Sal.在口腔鳞癌发生发展过程中可能起重要调控作用。

Objective By comparing the protein composition in the serum of patients with oral squamous cell carcinoma and that in the serum of healthy people, so as to obtain the protein changes in the serum of the patients. Methods Peripheral serum samples of 20 patients with oral squamous cell carcinoma and 20 healthy people were collected. Two-dimensional gel eleetrophoresis with immobi- lized pH gradients, mass spectrometry and bioinformaties analysis were employed to screen the differentially expressed proteins in ser- ums of patients with oral squamous cell carcinoma. Results Seven protein spots were significantly differentially expressed in serums of patients with oral squamous cell carcinoma compared with healthy people, and one of them was Chain B, T-To-T（High） Quaternary Transitions In Human Hemoglobin, which was lowly expressed in the serums of patients with oral squamous cell carcinoma. Conclusion Expression of Chain B, T-To-T（High） Quaternary Transitions In Human Hemoglobin：Deshis146beta Deoxy Low-Sal in peripheral serum may play an important role of modulation in the occurrence and development of oral squamous cell carcinoma.