Nd：YAG激光对口腔鳞癌CAL-27细胞迁移的影响

The effect of 1064 nm Nd: YAG laser irradiation on the migration of oral squamous cell carcinoma CAL-27 cells

目的探讨1064 nm Nd:YAG激光对口腔鳞癌CAL-27细胞迁移的影响。方法体外培养鳞癌CAL-27细胞,取处于对数生长期的细胞接种于24孔板中并继续培养24 h,分0、120、240、360、480 s五组接受激光照射,对应剂量为0、44.4、88.8、133.2、177.6 J/cm^2,划痕后分别于0、12、24、48 h在倒置显微镜下观察划痕变化并拍照,以研究激光照射对细胞迁移影响。结果低剂量激光照射后细胞划痕区域愈合程度提高,而高剂量激光照射后,细胞划痕区域愈合程度降低。结论低剂量Nd:YAG激光照射对鳞癌CAL-27细胞迁移有一定促进作用,而高剂量Nd:YAG激光照射则会对鳞癌CAL-27细胞的迁移产生明显抑制作用。

Objective To investigate the effect of 1064 nm Nd：YAG laser irradiation on migration of oral squamous cell carcinoma CAL-27 cells. Methods Squamous carcinoma CAL-27 cells were cultured in vitro, and cells in logarithmic growth phase were inocu- lated in 24-well plates and irradiated with 1064 nm Nd：YAG continuous wave laser for different time intervals （0, 120,240,360,480 s） with different corresponding doses （0, 44.4, 88.8, 133.2, 177.6 J/cm2 ）. After the specimens were scratched, the healing chan- ges of those scratches were observed under inverted microscope at 0, 12, 24, 48 h. Results After a low close of laser irradiation, the wound healing degree were improved, while after a high dose of laser irradiation, the degree of wound healing was reduced. Conclu- sions The squamous carcinoma CAL-27 cell migration can be stimulated by 1064 nm Nd ： YAG laser at a low intensity, while it can be inhibited by high intensity laser.