硬皮病皮损中热休克蛋白47、碱性成纤维细胞生长因子及Ⅰ型胶原蛋白的检测及意义

The expression and significance of HSP47,b FGF and COL-1 in scleroderma

目的:探讨热休克蛋白(HSP)47、碱性成纤维细胞生长因子(b FGF)及Ⅰ型胶原(COL-1)在硬皮病皮损中的表达及意义,分析三者在硬皮病发病机制中的作用及相互之间的关系。方法:采用免疫组化法检测早期硬皮病组(21例)、晚期硬皮病组(21例)及正常对照组(10例)皮肤石蜡包埋组织中HSP47、b FGF及COL-1的原位表达情况。结果:HSP47、b FGF及COL-1均弥漫表达于正常皮肤和硬皮病皮损的细胞质中。HSP47在早期硬皮病组和晚期硬皮病组中的表达均高于正常对照组(P<0.01),但三者分别在早、晚期硬皮病组中的表达比较,差异无统计学意义(P>0.05)。硬皮病皮损中HSP47与COL-1的表达呈正相关(r=0.579,P=0.000);b FGF与COL-1的表达呈正相关(r=0.379,P=0.000);HSP47与b FGF的表达也呈正相关(r=0.493,P=0.000)。结论:HSP47、b FGF及COL-1在早、晚期硬皮病皮损中表达均明显上调,HSP47、b FGF及COL-1可能参与硬皮病的病理纤维化进程。

Objectives： To investigate the expression and significances of HSP47, bFGF and COL-1 in patients with sclero- derma, and to explore their roles and correlation in the pathogenesis of scleroderma. Methods： The in situ expression of HSP47, bFGF and COL-1 was detected by immunohistochemical staining in earlier stage scleroderma group （21 cases）, later stage scleroderma group （21 cases） and normal control group （10 cases）. Results： Immunohistochemistry revealed that Hsp47, bFGF and COL-1 were diffusively expressed in the cytoplasm of normal and scleroderma skin. The expression levels of HSP47, bFGF and COL-1 were significantly higher in earlier and later stage scleroderma groups than in normal controls （P〈0.01 for all）, but were no significant differences between the earlier and later stage scleroderma groups （P〉0.05 for all）. In patients with scleroderma, the expression levels of COL-1 were positively correlated with HSP47 （r=0.579, P=0.000） and bFGF （r=0.379, P=0.000）. In addition, the expression levels of HSP47 was positively correlated with bFGF （r=0.493, P= 0.000）. Conclusion： The expression levels of HSP47, bFGF and COL-1 are significantly upregulated in patients with sclero- derma, and they may be involved in the pathogenesis of fibrosis in scleroderma.